Originally published as Genetics Published Articles Ahead of Print on October 16, 2004.

Genetics, Vol. 169, 1165-1167, February 2005, Copyright © 2005
doi:10.1534/genetics.104.035113

The Generation of Cloned Drosophila melanogaster

Department of Biology, Dalhousie University, Halifax, Nova Scotia B4H 4J1, Canada

1 Corresponding author: Department of Biology, Dalhousie University, Life Sciences Bldg., 1355 Oxford St., Halifax, Nova Scotia B3H 4J1, Canada.
E-mail: vlloyd{at}dal.ca

We report here the first successful use of embryonic nuclear transfer to create viable adult Drosophila melanogaster clones. Given the generation time, cost effectiveness, and relative ease of embryonic nuclear transplant in Drosophila, this method can provide an opportunity to further study the constraints on development imposed by transplanting determined or differentiated nuclei.