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Originally published as Genetics Published Articles Ahead of Print on September 30, 2004.
Genetics, Vol. 169, 77-89, January 2005, Copyright © 2005
doi:10.1534/genetics.104.034231
A Screen for Schizosaccharomyces pombe Mutants Defective in Rereplication Identifies New Alleles of rad4+, cut9+ and psf2+
Eliana B. Gómez*,
,1,
Vanessa T. Angeles*,1 and
Susan L. Forsburg*,,2
* Molecular and Cell Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037
Molecular and Computational Biology Section, University of Southern California, Los Angeles, California 90089-1340
2 Corresponding author: Molecular and Computational Biology Section, University of Southern California, 835 W. 37th St., SHS 464, Los Angeles, CA 90089-1340.
E-mail: forsburg{at}usc.edu
Fission yeast mutants defective in DNA replication have widely varying morphological phenotypes. We designed a screen for temperature-sensitive mutants defective in the process of replication regardless of morphology by isolating strains unable to rereplicate their DNA in the absence of cyclin B (Cdc13). Of the 42 rereplication-defective mutants analyzed, we were able to clone complementing plasmids for 10. This screen identified new alleles of the APC subunit cut9+, the initiation/checkpoint factor rad4+/cut5+, and the first mutant allele of psf2+, a subunit of the novel GINS replication complex. Other genes identified are likely to play general roles in gene expression and protein localization.
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