- THIS ARTICLE
- Full Text
- Full Text (PDF)
-
All Versions of this Article:
genetics.104.031146v1
169/1/325 most recent - Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Zhang, D.
- Articles by Cheng, Z.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Zhang, D.
- Articles by Cheng, Z.
Originally published as Genetics Published Articles Ahead of Print on September 15, 2004.
Genetics, Vol. 169, 325-335, January 2005, Copyright © 2005
doi:10.1534/genetics.104.031146
Molecular Cytogenetic Characterization of the Antirrhinum majus Genome
Dongfen Zhang*,1,
Qiuying Yang*,1,
Weidong Bao*,
Yu Zhang
,
Bin Han,
Yongbiao Xue* and
Zhukuan Cheng*,2
* Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China
National Center for Gene Research, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200233, China
2 Corresponding author: Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
E-mail: zkcheng{at}genetics.ac.cn
As a model system in classical plant genetics, the genus Antirrhinum has been well studied, especially in gametophytic self-incompatibility, flower development biology, and transposon-induced mutation. In contrast to the advances in genetic and molecular studies, little is known about Antirrhinum cytogenetics. In this study, we isolated two tandem repetitive sequences, CentA1 and CentA2, from the centromeric regions of Antirrhinum chromosomes. A standard karyotype has been established by anchoring these centromeric repeats on meiotic pachytene chromosome using FISH. An ideogram based on the DAPI-staining pattern of pachytene chromosomes was developed to depict the distribution of heterochromatin in the Antirrhinum majus genome. To integrate the genetic and chromosomal maps, we selected one or two molecular markers from each linkage group to screen an Antirrhinum transformation-competent artificial chromosome (TAC) library. These genetically anchored TAC clones were labeled as FISH probes to hybridize to pachytene chromosomes of A. majus. As a result, the relationship between chromosomes and the linkage groups (LGs) in Antirrhinum has been established.
This article has been cited by other articles:
 |
|
 |
|
  W. Zhang, C. Yi, W. Bao, B. Liu, J. Cui, H. Yu, X. Cao, M. Gu, M. Liu, and Z. Cheng The Transcribed 165-bp CentO Satellite Is the Major Functional Centromeric Element in the Wild Rice Species Oryza punctata Plant Physiology, September 1, 2005; 139(1): 306 - 315. [Abstract] [Full Text] [PDF]
|
|