Genetics, Vol. 168, 1937-1949, December 2004, Copyright © 2004
doi:10.1534/genetics.104.028423

The L-Type Cyclin CYL-1 and the Heat-Shock-Factor HSF-1 Are Required for Heat-Shock-Induced Protein Expression in Caenorhabditis elegans

Yvonne M. Hajdu-Cronin, Wen J. Chen and Paul W. Sternberg1

Howard Hughes Medical Institute and Division of Biology, California Institute of Technology, Pasadena, California 91125

1 Corresponding author: Biology 156-29, 1200 E. California Blvd., California Institute of Technology, Pasadena, CA 91125.
E-mail: pws{at}its.caltech.edu

In a screen for suppressors of activated GOA-1 (G{alpha}o) under the control of the hsp-16.2 heat-shock promoter, we identified three genetic loci that affected heat-shock-induced GOA-1 expression. The cyl-1 mutants are essentially wild type in appearance, while hsf-1 and sup-45 mutants have egg-laying defects. The hsf-1 mutation also causes a temperature-sensitive developmental arrest, and hsf-1 mutants have decreased life span. Western analysis indicated that mutations in all three loci suppressed the activated GOA-1 transgene by decreasing its expression. Heat-shock-induced expression of hsp-16.2 mRNA was reduced in cyl-1 mutants and virtually eliminated in hsf-1 and sup-45 mutants, as compared to wild-type expression. The mutations could also suppress other transgenes under heat-shock control. cyl-1 and sup-45, but not hsf-1, mutations suppressed a defect caused by a transgene not under heat-shock control, suggesting a role in general transcription or a post-transcriptional aspect of gene expression. hsf-1 encodes the C. elegans homolog of the human heat-shock factor HSF1, and cyl-1 encodes a cyclin most similar to cyclin L. We believe HSF-1 acts in heat-shock-inducible transcription and CYL-1 acts more generally in gene expression.




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