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Genetics, Vol. 168, 1805-1816, December 2004, Copyright © 2004
doi:10.1534/genetics.104.032912

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Competing Crossover Pathways Act During Meiosis in Saccharomyces cerevisiae

Juan Lucas Argueso, Jennifer Wanat, Zekeriyya Gemici and Eric Alani1

Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853

1 Corresponding author: Department of Molecular Biology and Genetics, 459 Biotechnology Bldg., Cornell University, Ithaca, NY 14853-2703.
E-mail: eea3{at}cornell.edu

In Saccharomyces cerevisiae the MSH4-MSH5, MLH1-MLH3, and MUS81-MMS4 complexes act to promote crossing over during meiosis. MSH4-MSH5, but not MUS81-MMS4, promotes crossovers that display interference. A role for MLH1-MLH3 in crossover control is less clear partly because mlh1{Delta} mutants retain crossover interference yet display a decrease in crossing over that is only slightly less severe than that seen in msh4{Delta} and msh5{Delta} mutants. We analyzed the effects of msh5{Delta}, mlh1{Delta}, and mms4{Delta} single, double, and triple mutants on meiotic crossing over at four consecutive genetic intervals on chromosome XV using newly developed computer software. mlh1{Delta} mms4{Delta} double mutants displayed the largest decrease in crossing over (13- to 15-fold) of all mutant combinations, yet these strains displayed relatively high spore viability (42%). In contrast, msh5{Delta} mms4{Delta} and msh5{Delta} mms4{Delta} mlh1{Delta} mutants displayed smaller decreases in crossing over (4- to 6-fold); however, spore viability (18–19%) was lower in these strains than in mlh1{Delta} mms4{Delta} strains. These data suggest that meiotic crossing over can occur in yeast through three distinct crossover pathways. In one pathway, MUS81-MMS4 promotes interference-independent crossing over; in a second pathway, both MSH4-MSH5 and MLH1-MLH3 promote interference-dependent crossovers. A third pathway, which appears to be repressed by MSH4-MSH5, yields deleterious crossovers.




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