- THIS ARTICLE
- Full Text
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Robertson, W. R.
- Articles by Sussman, M. R.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Robertson, W. R.
- Articles by Sussman, M. R.
Genetics, Vol. 168, 1677-1687, November 2004, Copyright © 2004
doi:10.1534/genetics.104.032326
An Arabidopsis thaliana Plasma Membrane Proton Pump Is Essential for Pollen Development
Whitney R. Robertson*,
Katherine Clark
,
Jeffery C. Young and
Michael R. Sussman*,
,1
* Program in Cellular and Molecular Biology, University of Wisconsin, Madison, Wisconsin 53706
Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706
Department of Biology, Western Washington University, Bellingham, Washington 98225
1 Corresponding author: Biotechnology Center, 425 Henry Mall, University of Wisconsin, Madison, WI 53706.
E-mail: msussman{at}facstaff.wisc.edu
The plasma membrane proton pump (H+-ATPase) found in plants and fungi is a P-type ATPase with a polypeptide sequence, structure, and in vivo function similar to the mammalian sodium pump (Na+, K+-ATPase). Despite its hypothetical importance for generating and maintaining the proton motive force that energizes the carriers and channels that underlie plant nutrition, genetic evidence for such a central function has not yet been reported. Using a reverse genetic approach for investigating each of the 11 isoforms in the Arabidopsis H+-ATPase (AHA) gene family, we found that one member, AHA3, is essential for pollen formation. A causative role for AHA3 in male gametogenesis was proven by complementation with a normal transgenic gene and rescue of the mutant phenotype back to wild type. We also investigated the requirement for phosphorylation of the penultimate threonine, which is found in most members of the AHA family and is thought to be involved in regulating catalytic activity. We demonstrated that a T948D mutant form of the AHA3 gene rescues the mutant phenotype in knockout AHA3 plants, but T948A does not, providing the first in planta evidence in support of the model in which phosphorylation of this amino acid is essential.
This article has been cited by other articles:
 |
|
 |
|
  R. Berthome, M. Thomasset, M. Maene, N. Bourgeois, N. Froger, and F. Budar pur4 Mutations Are Lethal to the Male, But Not the Female, Gametophyte and Affect Sporophyte Development in Arabidopsis Plant Physiology, June 1, 2008; 147(2): 650 - 660. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. W. Bock, D. Honys, J. M. Ward, S. Padmanaban, E. P. Nawrocki, K. D. Hirschi, D. Twell, and H. Sze Integrating Membrane Transport with Male Gametophyte Development and Function through Transcriptomics. Plant Physiology, April 1, 2006; 140(4): 1151 - 1168. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. I. Clark, C. Brooksbank, and J. Lomax It's All GO for Plant Scientists Plant Physiology, July 1, 2005; 138(3): 1268 - 1279. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. R. Baxter, J. C. Young, G. Armstrong, N. Foster, N. Bogenschutz, T. Cordova, W. A. Peer, S. P. Hazen, A. S. Murphy, and J. F. Harper A plasma membrane H+-ATPase is required for the formation of proanthocyanidins in the seed coat endothelium of Arabidopsis thaliana PNAS, February 15, 2005; 102(7): 2649 - 2654. [Abstract] [Full Text] [PDF]
|
|