- THIS ARTICLE
- Full Text
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Email this article to a friend
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Kruijt, M.
- Articles by de Wit, P. J. G. M.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Kruijt, M.
- Articles by de Wit, P. J. G. M.
Genetics, Vol. 168, 1655-1663, November 2004, Copyright © 2004
doi:10.1534/genetics.104.028985
Rearrangements in the Cf-9 Disease Resistance Gene Cluster of Wild Tomato Have Resulted in Three Genes That Mediate Avr9 Responsiveness
Marco Kruijt, Bas F. Brandwagt and Pierre J. G. M. de Wit1
Laboratory of Phytopathology, Wageningen University, Wageningen 6709 PD, The Netherlands
1 Corresponding author: Laboratory of Phytopathology, Wageningen University, Binnenhaven 5, 6709 PD Wageningen, The Netherlands.
E-mail: pierre.dewit{at}wur.nl
Cf resistance genes in tomato confer resistance to the fungal leaf pathogen Cladosporium fulvum. Both the well-characterized resistance gene Cf-9 and the related 9DC gene confer resistance to strains of C. fulvum that secrete the Avr9 protein and originate from the wild tomato species Lycopersicon pimpinellifolium. We show that 9DC and Cf-9 are allelic, and we have isolated and sequenced the complete 9DC cluster of L. pimpinellifolium LA1301. This 9DC cluster harbors five full-length Cf homologs, including orthologs of the most distal homologs of the Cf-9 cluster and three central 9DC genes. Two 9DC genes (9DC1 and 9DC2) have an identical coding sequence, whereas 9DC3 differs at its 3' terminus. From a detailed comparison of the 9DC and Cf-9 clusters, we conclude that the Cf-9 and Hcr9-9D genes from the Cf-9 cluster are ancestral to the first 9DC gene and that the three 9DC genes were generated by subsequent intra- and intergenic unequal recombination events. Thus, the 9DC cluster has undergone substantial rearrangements in the central region, but not at the ends. Using transient transformation assays, we show that all three 9DC genes confer Avr9 responsiveness, but that 9DC2 is likely the main determinant of Avr9 recognition in LA1301.
This article has been cited by other articles:
 |
|
 |
|
  E. F. Fradin, Z. Zhang, J. C. Juarez Ayala, C. D.M. Castroverde, R. N. Nazar, J. Robb, C.-M. Liu, and B. P.H.J. Thomma Genetic Dissection of Verticillium Wilt Resistance Mediated by Tomato Ve1 Plant Physiology, May 1, 2009; 150(1): 320 - 332. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. F. HANCOCK Contributions of Domesticated Plant Studies to our Understanding of Plant Evolution Ann. Bot., November 1, 2005; 96(6): 953 - 963. [Abstract] [Full Text] [PDF]
|
|
|