- THIS ARTICLE
- Full Text
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Yan, N.
- Articles by Macdonald, P. M.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Yan, N.
- Articles by Macdonald, P. M.
Genetics, Vol. 168, 1433-1442, November 2004, Copyright © 2004
doi:10.1534/genetics.104.033985
Genetic Interactions of Drosophila melanogaster arrest Reveal Roles for Translational Repressor Bruno in Accumulation of Gurken and Activity of Delta
Nan Yan and Paul M. Macdonald1
Institute for Cellular and Molecular Biology, Section of Molecular, Cell and Developmental Biology, University of Texas, Austin, Texas 78712-0159
1 Corresponding author: Section of Molecular, Cell and Developmental Biology, Institute for Cellular and Molecular Biology, University of Texas, 1 University Station, A-4800, Austin, TX 78712-0159.
E-mail: pmacdonald{at}mail.utexas.edu
arrest mutants have pleiotropic phenotypes, ranging from an early arrest of oogenesis to irregular embryonic segmentation defects. One function of arrest is in translational repression of oskar mRNA; this biochemical activity is presumed to be involved in other functions of arrest. To identify genes that could provide insight into how arrest contributes to translational repression or that may be targets for arrest-dependent translational control, we screened deficiency mutants for dominant modification of the arrest phenotype. Only four of the many deficiencies tested, which cover 
30% of the genome, modified the starting phenotype. One enhancer, identified fortuitously, is the Star gene. Star interaction with arrest results in excess Gurken protein, supporting the model that gurken is a target of repression. Two modifiers were mapped to individual genes. One is Lk6, which encodes a protein kinase predicted to regulate the rate-limiting initiation factor eIF4E. The second is Delta. The interaction between arrest and Delta mimics the phenotype of homozygous Delta mutants, suggesting that arrest could positively control Delta activity. Indeed, arrest mutants have significantly reduced levels of Delta protein at the interface of germline and follicle cells.
This article has been cited by other articles:
 |
|
 |
|
  C. Geng and P. M. Macdonald Imp Associates with Squid and Hrp48 and Contributes to Localized Expression of gurken in the Oocyte Mol. Cell. Biol., December 15, 2006; 26(24): 9508 - 9516. [Abstract] [Full Text] [PDF]
|
|