help button home button Genetics Mol Pharm
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Genetics, Vol. 168, 1337-1352, November 2004, Copyright © 2004
doi:10.1534/genetics.104.026369

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kiehart, D. P.
Right arrow Articles by Ashburner, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kiehart, D. P.
Right arrow Articles by Ashburner, M.

Drosophila crinkled, Mutations of Which Disrupt Morphogenesis and Cause Lethality, Encodes Fly Myosin VIIA

Daniel P. Kiehart*,1, Josef D. Franke*, Mark K. Chee*, R. A. Montague*, Tung-ling Chen{dagger}, John Roote{ddagger} and Michael Ashburner{ddagger}

* Department of Biology, Duke University, Durham, North Carolina 27708-1000
{dagger} Department of Cell Biology and Anatomy, Finch University of Health Sciences, Chicago Medical School, North Chicago, Illinois 60064
{ddagger} Department of Genetics, University of Cambridge, Cambridge, United Kingdom CB2 3EH

1 Corresponding author: DCMB Group, Department of Biology, Rm. B330G, LSRC Bldg., Duke University, Box 91000, Research Dr., Durham, NC 27708-1000.
E-mail: dkiehart{at}duke.edu

Myosin VIIs provide motor function for a wide range of eukaryotic processes. We demonstrate that mutations in crinkled (ck) disrupt the Drosophila myosin VIIA heavy chain. The ck/myoVIIA protein is present at a low level throughout fly development and at the same level in heads, thoraxes, and abdomens. Severe ck alleles, likely to be molecular nulls, die as embryos or larvae, but all allelic combinations tested thus far yield a small fraction of adult "escapers" that are weak and infertile. Scanning electron microscopy shows that escapers have defects in bristles and hairs, indicating that this motor protein plays a role in the structure of the actin cytoskeleton. We generate a homology model for the structure of the ck/myosin VIIA head that indicates myosin VIIAs, like myosin IIs, have a spectrin-like, SH3 subdomain fronting their N terminus. In addition, we establish that the two myosin VIIA FERM repeats share high sequence similarity with only the first two subdomains of the three-lobed structure that is typical of canonical FERM domains. Nevertheless, the ~100 and ~75 amino acids that follow the first two lobes of the first and second FERM domains are highly conserved among myosin VIIs, suggesting that they compose a conserved myosin tail homology 7 (MyTH7) domain that may be an integral part of the FERM domain or may function independently of it. Together, our data suggest a key role for ck/myoVIIA in the formation of cellular projections and other actin-based functions required for viability.




This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
H. M. Prosser, A. K. Rzadzinska, K. P. Steel, and A. Bradley
Mosaic Complementation Demonstrates a Regulatory Role for Myosin VIIa in Actin Dynamics of Stereocilia
Mol. Cell. Biol., March 1, 2008; 28(5): 1702 - 1712.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
R. Liu, S. Woolner, J. E. Johndrow, D. Metzger, A. Flores, and S. M. Parkhurst
Sisyphus, the Drosophila myosin XV homolog, traffics within filopodia transporting key sensory and adhesion cargos
Development, January 1, 2008; 135(1): 53 - 63.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
N. Ren, J. Charlton, and P. N. Adler
The flare Gene, Which Encodes the AIP1 Protein of Drosophila, Functions to Regulate F-Actin Disassembly in Pupal Epidermal Cells
Genetics, August 1, 2007; 176(4): 2223 - 2234.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. B. Bohil, B. W. Robertson, and R. E. Cheney
Myosin-X is a molecular motor that functions in filopodia formation
PNAS, August 15, 2006; 103(33): 12411 - 12416.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
Y. Mao, C. Rauskolb, E. Cho, W.-L. Hu, H. Hayter, G. Minihan, F. N. Katz, and K. D. Irvine
Dachs: an unconventional myosin that functions downstream of Fat to regulate growth, affinity and gene expression in Drosophila
Development, July 1, 2006; 133(13): 2539 - 2551.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Y. Yang, M. Kovacs, T. Sakamoto, F. Zhang, D. P. Kiehart, and J. R. Sellers
Dimerized Drosophila myosin VIIa: A processive motor
PNAS, April 11, 2006; 103(15): 5746 - 5751.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
N. Ren, B. He, D. Stone, S. Kirakodu, and P. N. Adler
The shavenoid Gene of Drosophila Encodes a Novel Actin Cytoskeleton Interacting Protein That Promotes Wing Hair Morphogenesis
Genetics, March 1, 2006; 172(3): 1643 - 1653.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Yang, M. Kovacs, Q. Xu, J. B. Anderson, and J. R. Sellers
Myosin VIIB from Drosophila Is a High Duty Ratio Motor
J. Biol. Chem., September 16, 2005; 280(37): 32061 - 32068.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2004 by the Genetics Society of America.