- THIS ARTICLE
- Full Text
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Email this article to a friend
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Nabeshima, K.
- Articles by Hillers, K. J.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Nabeshima, K.
- Articles by Hillers, K. J.
Genetics, Vol. 168, 1275-1292, November 2004, Copyright © 2004
doi:10.1534/genetics.104.030700
Chromosome-Wide Regulation of Meiotic Crossover Formation in Caenorhabditis elegans Requires Properly Assembled Chromosome Axes
Kentaro Nabeshima, Anne M. Villeneuve1 and Kenneth J. Hillers2
Department of Developmental Biology and Department of Genetics, Stanford University School of Medicine, Stanford, California 94305
1 Corresponding author: Department of Developmental Biology, Stanford University School of Medicine, 279 Campus Dr., B300, Beckman Center, Stanford, CA 94305-5329.
E-mail: villen{at}cmgm.stanford.edu
Most sexually reproducing organisms depend on the regulated formation of crossovers, and the consequent chiasmata, to accomplish successful segregation of homologous chromosomes at the meiosis I division. A robust, chromosome-wide crossover control system limits chromosome pairs to one crossover in most meioses in the nematode Caenorhabditis elegans; this system has been proposed to rely on structural integrity of meiotic chromosome axes. Here, we test this hypothesis using a mutant, him-3(me80), that assembles reduced levels of meiosis-specific axis component HIM-3 along cohesin-containing chromosome axes. Whereas pairing, synapsis, and crossing over are eliminated when HIM-3 is absent, the him-3(me80) mutant supports assembly of synaptonemal complex protein SYP-1 along some paired chromosomes, resulting in partial competence for chiasma formation. We present both genetic and cytological evidence indicating that the him-3(me80) mutation leads to an increased incidence of meiotic products with two crossovers. These results indicate that limiting the amount of a major axis component results in a reduced capacity to communicate the presence of a (nascent) crossover and/or to discourage others in response.
This article has been cited by other articles:
 |
|
 |
|
  E. Martinez-Perez, M. Schvarzstein, C. Barroso, J. Lightfoot, A. F. Dernburg, and A. M. Villeneuve Crossovers trigger a remodeling of meiotic chromosome axis composition that is linked to two-step loss of sister chromatid cohesion Genes & Dev., October 15, 2008; 22(20): 2886 - 2901. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. G. Y. Lim, R. R. W. Stine, and J. L. Yanowitz Domain-Specific Regulation of Recombination in Caenorhabditis elegans in Response to Temperature, Age and Sex Genetics, October 1, 2008; 180(2): 715 - 726. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. J. Tsai, D. G. Mets, M. R. Albrecht, P. Nix, A. Chan, and B. J. Meyer Meiotic crossover number and distribution are regulated by a dosage compensation protein that resembles a condensin subunit Genes & Dev., January 15, 2008; 22(2): 194 - 211. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Jantsch, L. Tang, P. Pasierbek, A. Penkner, S. Nayak, A. Baudrimont, T. Schedl, A. Gartner, and J. Loidl Caenorhabditis elegans prom-1 Is Required for Meiotic Prophase Progression and Homologous Chromosome Pairing Mol. Biol. Cell, December 1, 2007; 18(12): 4911 - 4920. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Smolikov, A. Eizinger, K. Schild-Prufert, A. Hurlburt, K. McDonald, J. Engebrecht, A. M. Villeneuve, and M. P. Colaiacovo SYP-3 Restricts Synaptonemal Complex Assembly to Bridge Paired Chromosome Axes During Meiosis in Caenorhabditis elegans Genetics, August 1, 2007; 176(4): 2015 - 2025. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. de Boer, A. J. J. Dietrich, C. Hoog, P. Stam, and C. Heyting Meiotic interference among MLH1 foci requires neither an intact axial element structure nor full synapsis J. Cell Sci., March 1, 2007; 120(5): 731 - 736. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Wang and C. Hoog Structural damage to meiotic chromosomes impairs DNA recombination and checkpoint control in mammalian oocytes J. Cell Biol., May 22, 2006; 173(4): 485 - 495. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Martinez-Perez and A. M. Villeneuve HTP-1-dependent constraints coordinate homolog pairing and synapsis and promote chiasma formation during C. elegans meiosis Genes & Dev., November 15, 2005; 19(22): 2727 - 2743. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Couteau and M. Zetka HTP-1 coordinates synaptonemal complex assembly with homolog alignment during meiosis in C. elegans Genes & Dev., November 15, 2005; 19(22): 2744 - 2756. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Hammarlund, M. W. Davis, H. Nguyen, D. Dayton, and E. M. Jorgensen Heterozygous Insertions Alter Crossover Distribution but Allow Crossover Interference in Caenorhabditis elegans Genetics, November 1, 2005; 171(3): 1047 - 1056. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Nabeshima, A. M. Villeneuve, and M. P. Colaiacovo Crossing over is coupled to late meiotic prophase bivalent differentiation through asymmetric disassembly of the SC J. Cell Biol., February 28, 2005; 168(5): 683 - 689. [Abstract] [Full Text] [PDF]
|
|