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Genetics, Vol. 168, 723-731, October 2004, Copyright © 2004
doi:10.1534/genetics.104.029876

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Enolase and Glycolytic Flux Play a Role in the Regulation of the Glucose Permease Gene RAG1 of Kluyveromyces lactis

Marc Lemaire1 and Micheline Wésolowski-Louvel

UMR 5122 Microbiologie et Génétique, Université Claude Bernard Lyon 1, 69622 Villeurbanne, France

1 Corresponding author: Unité Microbiologie et Génétique UMR 5122 CNRS/UCBL/INSA, Université Claude Bernard, Bâtiment Lwoff, 43, Blvd. du 11 Novembre 1918, 69622 Villeurbanne Cédex, France.
E-mail: mlemaire{at}univ-lyon1.fr

We isolated a mutant, rag17, which is impaired in glucose induction of expression of the major glucose transporter gene RAG1. The RAG17 gene encodes a protein 87% identical to S. cerevisiae enolases (Eno1 and Eno2). The Kleno null mutant showed no detectable enolase enzymatic activity and has severe growth defects on glucose and gluconeogenic carbon sources, indicating that K. lactis has a single enolase gene. In addition to RAG1, the transcription of several glycolytic genes was also strongly reduced in the {Delta}Kleno mutant. Moreover, the defect in RAG1 expression was observed in other mutants of the glycolytic pathway (hexokinase and phosphoglycerate kinase). Therefore, it seems that the enolase and a functional glycolytic flux are necessary for induction of expression of the Rag1 glucose permease in K. lactis.




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