help button home button Genetics AJP: Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Genetics, Vol. 168, 651-663, October 2004, Copyright © 2004
doi:10.1534/genetics.104.034827

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Miftahudin,
Right arrow Articles by Gustafson, J. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Miftahudin,
Right arrow Articles by Gustafson, J. P.

Analysis of Expressed Sequence Tag Loci on Wheat Chromosome Group 4

Miftahudin*,{dagger}, K. Ross{ddagger}, X.-F. Ma*, A. A. Mahmoud*, J. Layton*, M. A. Rodriguez Milla*, T. Chikmawati*,{dagger}, J. Ramalingam*, O. Feril*, M. S. Pathan*, G. Surlan Momirovic*,§, S. Kim*, K. Chema*, P. Fang*, L. Haule*, H. Struxness*, J. Birkes*, C. Yaghoubian*, R. Skinner*, J. McAllister*, V. Nguyen*, L. L. Qi, B. Echalier, B. S. Gill, A. M. Linkiewicz&,1, J. Dubcovsky&, E. D. Akhunov&, J. Dvorák&, M. Dilbirligi**, K. S. Gill**, J. H. Peng{dagger}{dagger}, N. L. V. Lapitan{dagger}{dagger}, C. E. Bermudez-Kandianis{ddagger}{ddagger}, M. E. Sorrells{ddagger}{ddagger}, K. G. Hossain§§, V. Kalavacharla§§, S. F. Kianian§§, G. R. Lazo¶¶, S. Chao&&,2, O. D. Anderson¶¶, J. Gonzalez-Hernandez***,3, E. J. Conley***, J. A. Anderson***, D.-W. Choi{dagger}{dagger}{dagger},4, R. D. Fenton{dagger}{dagger}{dagger}, T. J. Close{dagger}{dagger}{dagger}, P. E. McGuire&&, C. O. Qualset&&, H. T. Nguyen* and J. P. Gustafson{ddagger},5

* Department of Agronomy, University of Missouri, Columbia, Missouri 65211
{dagger} Department of Biology, Bogor Agricultural University, Bogor 16144, Indonesia
{ddagger} USDA-ARS Plant Genetics Research Unit, Department of Agronomy, University of Missouri, Columbia, Missouri 65211
§ Department of Plant Genetics and Breeding, University of Belgrade, 11080 Belgrade, Yugoslavia
Department of Plant Pathology, Wheat Genetics Resource Center, Kansas State University, Manhattan, Kansas 66506-5502
& Department of Agronomy and Range Science, University of California, Davis, California 95616
** Department of Crop and Soil Sciences, Washington State University, Pullman, Washington 99164-6420
{dagger}{dagger} Department of Soil and Crop Sciences, Colorado State University, Fort Collins, Colorado 80523-1170
{ddagger}{ddagger} Department of Plant Breeding, Cornell University, Ithaca, New York 14853
§§ Department of Plant Sciences, North Dakota State University, Fargo, North Dakota 58105
¶¶ USDA-ARS Western Regional Research Center, Albany, California 94710-1105
&& Genetic Resources Conservation Program, University of California, Davis, California 95616
*** Department of Agronomy and Plant Genetics, University of Minnesota, St Paul, Minnesota 55108
{dagger}{dagger}{dagger} Department of Botany and Plant Sciences, University of California, Riverside, California 92521

5 Corresponding author: USDA-ARS, 206 Curtis Hall, University of Missouri, Columbia, MO 65211.
E-mail: pgus{at}missouri.edu

A total of 1918 loci, detected by the hybridization of 938 expressed sequence tag unigenes (ESTs) from 26 Triticeae cDNA libraries, were mapped to wheat (Triticum aestivum L.) homoeologous group 4 chromosomes using a set of deletion, ditelosomic, and nulli-tetrasomic lines. The 1918 EST loci were not distributed uniformly among the three group 4 chromosomes; 41, 28, and 31% mapped to chromosomes 4A, 4B, and 4D, respectively. This pattern is in contrast to the cumulative results of EST mapping in all homoeologous groups, as reported elsewhere, that found the highest proportion of loci mapped to the B genome. Sixty-five percent of these 1918 loci mapped to the long arms of homoeologous group 4 chromosomes, while 35% mapped to the short arms. The distal regions of chromosome arms showed higher numbers of loci than the proximal regions, with the exception of 4DL. This study confirmed the complex structure of chromosome 4A that contains two reciprocal translocations and two inversions, previously identified. An additional inversion in the centromeric region of 4A was revealed. A consensus map for homoeologous group 4 was developed from 119 ESTs unique to group 4. Forty-nine percent of these ESTs were found to be homoologous to sequences on rice chromosome 3, 12% had matches with sequences on other rice chromosomes, and 39% had no matches with rice sequences at all. Limited homology (only 26 of the 119 consensus ESTs) was found between wheat ESTs on homoeologous group 4 and the Arabidopsis genome. Forty-two percent of the homoeologous group 4 ESTs could be classified into functional categories on the basis of blastX searches against all protein databases.




This article has been cited by other articles:


Home page
 GeneticsHome page
N. C. Collins, N. J. Shirley, M. Saeed, M. Pallotta, and J. P. Gustafson
An ALMT1 Gene Cluster Controlling Aluminum Tolerance at the Alt4 Locus of Rye (Secale cereale L.)
Genetics, May 1, 2008; 179(1): 669 - 682.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
J. Salse, S. Bolot, M. Throude, V. Jouffe, B. Piegu, U. M. Quraishi, T. Calcagno, R. Cooke, M. Delseny, and C. Feuillet
Identification and Characterization of Shared Duplications between Rice and Wheat Provide New Insight into Grass Genome Evolution
PLANT CELL, January 1, 2008; 20(1): 11 - 24.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
S. Cho, D. F. Garvin, and G. J. Muehlbauer
Transcriptome Analysis and Physical Mapping of Barley Genes in Wheat-Barley Chromosome Addition Lines
Genetics, February 1, 2006; 172(2): 1277 - 1285.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
G. R. Lazo, S. Chao, D. D. Hummel, H. Edwards, C. C. Crossman, N. Lui, D. E. Matthews, V. L. Carollo, D. L. Hane, F. M. You, et al.
Development of an Expressed Sequence Tag (EST) Resource for Wheat (Triticum aestivum L.): EST Generation, Unigene Analysis, Probe Selection and Bioinformatics for a 16,000-Locus Bin-Delineated Map
Genetics, October 1, 2004; 168(2): 585 - 593.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
D. Zhang, D. W. Choi, S. Wanamaker, R. D. Fenton, A. Chin, M. Malatrasi, Y. Turuspekov, H. Walia, E. D. Akhunov, P. Kianian, et al.
Construction and Evaluation of cDNA Libraries for Large-Scale Expressed Sequence Tag Sequencing in Wheat (Triticum aestivum L.)
Genetics, October 1, 2004; 168(2): 595 - 608.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
L. L. Qi, B. Echalier, S. Chao, G. R. Lazo, G. E. Butler, O. D. Anderson, E. D. Akhunov, J. Dvorak, A. M. Linkiewicz, A. Ratnasiri, et al.
A Chromosome Bin Map of 16,000 Expressed Sequence Tag Loci and Distribution of Genes Among the Three Genomes of Polyploid Wheat
Genetics, October 1, 2004; 168(2): 701 - 712.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2004 by the Genetics Society of America.