Genetics, Vol. 168, 625-637, October 2004, Copyright © 2004
doi:10.1534/genetics.104.034801

A 2600-Locus Chromosome Bin Map of Wheat Homoeologous Group 2 Reveals Interstitial Gene-Rich Islands and Colinearity With Rice

E. J. Conley^*, V. Nduati^*, J. L. Gonzalez-Hernandez^*,1, A. Mesfin^*, M. Trudeau-Spanjers^*, S. Chao^,2, G. R. Lazo, D. D. Hummel, O. D. Anderson, L. L. Qi, B. S. Gill, B. Echalier, A. M. Linkiewicz^,3, J. Dubcovsky, E. D. Akhunov, J. Dvoák, J. H. Peng^¶, N. L. V. Lapitan^¶, M. S. Pathan^&, H. T. Nguyen^&, X.-F. Ma^&, Miftahudin^&, J. P. Gustafson^**, R. A. Greene, M. E. Sorrells, K. G. Hossain, V. Kalavacharla, S. F. Kianian, D. Sidhu, M. Dilbirligi, K. S. Gill, D. W. Choi^¶¶,4, R. D. Fenton^¶¶, T. J. Close^¶¶, P. E. McGuire^&&, C. O. Qualset^&& and J. A. Anderson^*,5

^* Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, Minnesota 55108
U.S. Department of Agriculture-Agricultural Research Center (USDA-ARS) Western Regional Research Center, Albany, California 94710-1105
Department of Plant Pathology, Wheat Genetics Resource Center, Kansas State University, Manhattan, Kansas 66506-5502
Department of Agronomy and Range Science, University of California, Davis, California 95616
^¶ Department of Soil and Crop Sciences, Colorado State University, Fort Collins, Colorado 80523-1170
^& Department of Agronomy, University of Missouri, Columbia, Missouri 65211
^** USDA-ARS Plant Genetics Research Unit, Department of Agronomy, University of Missouri, Columbia, Missouri 65211
Department of Plant Breeding, Cornell University, Ithaca, New York 14853
Department of Plant Sciences, North Dakota State University, Fargo, North Dakota 58105
Department of Crop and Soil Sciences, Washington State University, Pullman, Washington 99164-6420
^¶¶ Department of Botany and Plant Sciences, University of California, Riverside, California 92521
^&& Genetic Resources Conservation Program, University of California, Davis, California 95616

⁵ Corresponding author: Department of Agronomy and Plant Genetics, 411 Borlaug Hall, University of Minnesota, St. Paul, MN 55108.
E-mail: ander319{at}umn.edu

The complex hexaploid wheat genome offers many challenges for genomics research. Expressed sequence tags facilitate the analysis of gene-coding regions and provide a rich source of molecular markers for mapping and comparison with model organisms. The objectives of this study were to construct a high-density EST chromosome bin map of wheat homoeologous group 2 chromosomes to determine the distribution of ESTs, construct a consensus map of group 2 ESTs, investigate synteny, examine patterns of duplication, and assess the colinearity with rice of ESTs assigned to the group 2 consensus bin map. A total of 2600 loci generated from 1110 ESTs were mapped to group 2 chromosomes by Southern hybridization onto wheat aneuploid chromosome and deletion stocks. A consensus map was constructed of 552 ESTs mapping to more than one group 2 chromosome. Regions of high gene density in distal bins and low gene density in proximal bins were found. Two interstitial gene-rich islands flanked by relatively gene-poor regions on both the short and long arms and having good synteny with rice were discovered. The map locations of two ESTs indicated the possible presence of a small pericentric inversion on chromosome 2B. Wheat chromosome group 2 was shown to share syntenous blocks with rice chromosomes 4 and 7.

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