help button home button Genetics J Clin Inv
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Genetics, Vol. 168, 89-101, September 2004, Copyright © 2004
doi:10.1534/genetics.102.012708

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Radford, S. J.
Right arrow Articles by Keeney, J. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Radford, S. J.
Right arrow Articles by Keeney, J. B.

Increase in Ty1 cDNA Recombination in Yeast sir4 Mutant Strains at High Temperature

Sarah J. Radford1, Meredith L. Boyle2, Catherine J. Sheely, Joel Graham3, Daniel P. Haeusser4, Leigh Zimmerman and Jill B. Keeney5

Department of Biology, Juniata College, Huntingdon, Pennsylvania 16652

5 Corresponding author: Department of Biology, Juniata College, 1700 Moore St., Huntingdon, PA 16652.
E-mail: keeney{at}juniata.edu

Transposition of the Ty1 element of the yeast Saccharomyces cerevisiae is temperature sensitive. We have identified a null allele of the silent information regulator gene SIR4 as a host mutant that allows for transposition at high temperature. We show that the apparent increase in transposition activity in sir4 mutant strains at high temperature is dependent on the RAD52 gene and is thus likely resulting from an increase in Ty1 cDNA recombination, rather than in IN-mediated integration. General cellular recombination is not increased at high temperature, suggesting that the increase in recombination at high temperature in sir4 mutants is specific for Ty1 cDNA. Additionally, this high-temperature Ty1 recombination was found to be dependent on functional Sir2p and Sir3p. We speculate that the increase in recombination seen in sir4 mutants at high temperature may be due to changes in chromatin structure or Ty1 interactions with chromosomal structures resulting in higher recombination rates.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2004 by the Genetics Society of America.