Genetics, Vol. 168, 553-557, September 2004, Copyright © 2004
doi:10.1534/genetics.104.030551

A Novel Yeast Mutation, rad52-L89F, Causes a Specific Defect in Rad51-Independent Recombination That Correlates With a Reduced Ability of Rad52-L89F to Interact With Rad59

Felipe Cortés-Ledesma, Francisco Malagón1 and Andrés Aguilera2

Departamento de Genética, Facultad de Biología, Universidad de Sevilla, 41012 Sevilla, Spain

2 Corresponding author: Departamento de Genética, Facultad de Biología, Universidad de Sevilla, Avd. Reina Mercedes 6, 41012 Sevilla, Spain.
E-mail: aguilo{at}us.es

We isolated a novel rad52 mutation, rad52-L89F, which specifically impairs recombination in rad51{Delta} cells. rad52-L89F displays phenotypes similar to rad59{Delta} and encodes a mutant protein impaired in its ability to interact with Rad59. These results support the idea that Rad59 acts in homologous recombination via physical interaction with Rad52.




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