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Genetics, Vol. 167, 1643-1661, August 2004, Copyright © 2004
doi:10.1534/genetics.104.029652
High-Density Genetic Linkage Maps of Phytophthora infestans Reveal Trisomic Progeny and Chromosomal Rearrangements
Theo van der Lee1, Antonino Testa2, Andrea Robold3, John van ‘t Klooster and Francine Govers4
Laboratory of Phytopathology, Wageningen University, and Graduate School of Experimental Plant Sciences, 6709 PD Wageningen, The Netherlands
4 Corresponding author: Laboratory of Phytopathology, Wageningen University, Binnenhaven 5, 6709 PD Wageningen, The Netherlands.
E-mail: francine.govers{at}wur.nl
Detailed analysis of the inheritance of molecular markers was performed in the oomycete plant pathogen Phytophthora infestans. Linkage analysis in the sexual progeny of two Dutch field isolates (cross 71) resulted in a high-density map containing 508 markers on 13 major and 10 minor linkage groups. The map showed strong clustering of markers, particularly of markers originating from one parent, and dissimilarity between the parental isolates on linkage group III in the vicinity of the mating-type locus, indicating a chromosomal translocation. A second genetic map, constructed by linkage analysis in sexual progeny of two Mexican isolates (cross 68), contained 363 markers and is thus less dense than the cross 71 map. For some linkage groups the two independent linkage maps could be aligned, but sometimes markers appeared to be in a different order, or not linked at all, indicating chromosomal rearrangements between genotypes. Graphical genotyping showed that some progeny contained three copies of a homologous linkage group. This trisomy was found for several linkage groups in both crosses. Together, these analyses suggest a genome with a high degree of flexibility, which may have implications for evolution of new races and resistance development to crop protection agents.
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