Genetics, Vol. 167, 797-813, June 2004, Copyright © 2004
doi:10.1534/genetics.104.026658

The DrosDel Collection

A Set of P-Element Insertions for Generating Custom Chromosomal Aberrations in Drosophila melanogaster

Edward Ryder^*, Fiona Blows^*, Michael Ashburner^*, Rosa Bautista-Llacer^*, Darin Coulson^*, Jenny Drummond^*, Jane Webster^*, David Gubb^*, Nicola Gunton^*, Glynnis Johnson^*, Cahir J. O'Kane^*, David Huen^*, Punita Sharma^*, Zoltan Asztalos^*, Heiko Baisch, Janet Schulze, Maria Kube, Kathrin Kittlaus, Gunter Reuter, Peter Maroy, Janos Szidonya, Åsa Rasmuson-Lestander, Karin Ekström, Barry Dickson^**, Christoph Hugentobler, Hugo Stocker, Ernst Hafen, Jean Antoine Lepesant, Gert Pflugfelder, Martin Heisenberg^***, Bernard Mechler, Florenci Serras, Montserrat Corominas, Stephan Schneuwly, Thomas Preat^****, John Roote^* and Steven Russell^*,1

^* Department of Genetics, University of Cambridge, Cambridge CB2 3EH, United Kingdom
Institute of Genetics, Martin Luther University, D-06120 Halle, Germany
Department of Molecular Biology, Umeå University, S-901 87 Umeå, Sweden
Department of Genetics and Molecular Biology, University of Szeged, 6726 Szeged, Hungary
^** Institute of Molecular Biotechnology, Austrian Academy of Sciences, A-1030 Vienna, Austria
Zoologisches Institut der Universitat Zurich, 8057 Zurich, Switzerland
Biologie du Developpement, CNRS, Institute Jacques Monod, FR-75251 Paris, France
Institut für Genetik, Universität Mainz, 55128 Mainz, Germany
^*** Lehrstuhl für Genetik, Universität Würzburg, Am Hubland, 97074 Würzburg, Germany
Department of Developmental Genetics, A040, Deutsches Krebsforschungszentrum, D-69120 Heidelberg, Germany
Departament de Genetica, Facultat de Biologia, Universitat de Barcelona, 08028 Barcelona, Spain
Institute of Zoology, University of Regensburg, D-93040 Regensburg, Germany
^**** Développement, Evolution, Plasticité du Système Nerveux, CNRS, 91190 Gif-sur-Yvette, France

¹ Corresponding author: Department of Genetics, University of Cambridge, Downing St., Cambridge CB2 3EH, United Kingdom.
E-mail: s.russell{at}gen.cam.ac.uk

We describe a collection of P-element insertions that have considerable utility for generating custom chromosomal aberrations in Drosophila melanogaster. We have mobilized a pair of engineered P elements, p{RS3} and p{RS5}, to collect 3243 lines unambiguously mapped to the Drosophila genome sequence. The collection contains, on average, an element every 35 kb. We demonstrate the utility of the collection for generating custom chromosomal deletions that have their end points mapped, with base-pair resolution, to the genome sequence. The collection was generated in an isogenic strain, thus affording a uniform background for screens where sensitivity to genetic background is high. The entire collection, along with a computational and genetic toolbox for designing and generating custom deletions, is publicly available. Using the collection it is theoretically possible to generate >12,000 deletions between 1 bp and 1 Mb in size by simple eye color selection. In addition, a further 37,000 deletions, selectable by molecular screening, may be generated. We are now using the collection to generate a second-generation deficiency kit that is precisely mapped to the genome sequence.

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