help button home button Genetics AJP: Advances PE
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Genetics, Vol. 167, 619-631, June 2004, Copyright © 2004
doi:10.1534/genetics.103.023408

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Tables
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Marra, R. E.
Right arrow Articles by Mitchell, T. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Marra, R. E.
Right arrow Articles by Mitchell, T. G.

A Genetic Linkage Map of Cryptococcus neoformans variety neoformans Serotype D (Filobasidiella neoformans)

Robert E. Marra*,{dagger},1, Johnny C. Huang*, Eula Fung{ddagger}, Kirsten Nielsen*, Joseph Heitman*,§, Rytas Vilgalys*,{dagger} and Thomas G. Mitchell*

* Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina 27710
§ Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina 27710
{dagger} Department of Biology, Duke University, Durham, North Carolina 27710
{ddagger} Stanford Genome Technology Center, Palo Alto, California 94304

1 Corresponding author: Department of Plant Pathology and Ecology, Connecticut Agricultural Experiment Station, PO Box 1106, New Haven, CT 06504.
E-mail: robert.marra{at}po.state.ct.us

To construct a genetic linkage map of the heterothallic yeast, Cryptococcus neoformans (Filobasidiella neoformans), we crossed two mating-compatible strains and analyzed 94 progeny for the segregation of 301 polymorphic markers, consisting of 228 restriction site polymorphisms, 63 microsatellites, two indels, and eight mating-type (MAT)-associated markers. All but six markers showed no significant (P < 0.05) segregation distortion. At a minimum LOD score of 6.0 and a maximum recombination frequency of 0.30, 20 linkage groups were resolved, resulting in a map length of ~1500 cM. Average marker density is 5.4 cM (range 1–28.7 cM). Hybridization of selected markers to blots of electrophoretic karyotypes unambiguously assigned all linkage groups to chromosomes and led us to conclude that the C. neoformans genome is ~20.2 Mb, comprising 14 chromosomes ranging in size from 0.8 to 2.3 Mb, with a ratio of ~13.2 kb/cM averaged across the genome. However, only 2 of 12 ungrouped markers hybridized to chromosome 10. The hybridizations revealed at least one possible reciprocal translocation involving chromosomes 8, 9, and 12. This map has been critical to genome sequence assembly and will be essential for future studies of quantitative trait inheritance.




This article has been cited by other articles:


Home page
 Eukaryot CellHome page
J. C. Rutherford, X. Lin, K. Nielsen, and J. Heitman
Amt2 Permease Is Required To Induce Ammonium-Responsive Invasive Growth and Mating in Cryptococcus neoformans
Eukaryot. Cell, February 1, 2008; 7(2): 237 - 246.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
Y.-S. Bahn, S. Geunes-Boyer, and J. Heitman
Ssk2 Mitogen-Activated Protein Kinase Kinase Kinase Governs Divergent Patterns of the Stress-Activated Hog1 Signaling Pathway in Cryptococcus neoformans
Eukaryot. Cell, December 1, 2007; 6(12): 2278 - 2289.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
S. Sun and J. Xu
Genetic Analyses of a Hybrid Cross Between Serotypes A and D Strains of the Human Pathogenic Fungus Cryptococcus neoformans
Genetics, November 1, 2007; 177(3): 1475 - 1486.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
M. Cogliati, M. C. Esposto, G. Liberi, A. M. Tortorano, and M. A. Viviani
Cryptococcus neoformans Typing by PCR Fingerprinting Using (GACA)4 Primers Based on C. neoformans Genome Project Data
J. Clin. Microbiol., October 1, 2007; 45(10): 3427 - 3430.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
N. Backstrom, M. Brandstrom, L. Gustafsson, A. Qvarnstrom, H. Cheng, and H. Ellegren
Genetic Mapping in a Natural Population of Collared Flycatchers (Ficedula albicollis): Conserved Synteny but Gene Order Rearrangements on the Avian Z Chromosome
Genetics, September 1, 2006; 174(1): 377 - 386.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
A. P. Litvintseva, R. Thakur, R. Vilgalys, and T. G. Mitchell
Multilocus Sequence Typing Reveals Three Genetic Subpopulations of Cryptococcus neoformans var. grubii (Serotype A), Including a Unique Population in Botswana
Genetics, April 1, 2006; 172(4): 2223 - 2238.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
K. Nielsen, R. E. Marra, F. Hagen, T. Boekhout, T. G. Mitchell, G. M. Cox, and J. Heitman
Interaction Between Genetic Background and the Mating-Type Locus in Cryptococcus neoformans Virulence Potential
Genetics, November 1, 2005; 171(3): 975 - 983.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
Y.-S. Bahn, K. Kojima, G. M. Cox, and J. Heitman
Specialization of the HOG Pathway and Its Impact on Differentiation and Virulence of Cryptococcus neoformans
Mol. Biol. Cell, May 1, 2005; 16(5): 2285 - 2300.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
J. A. Fraser, J. C. Huang, R. Pukkila-Worley, J. A. Alspaugh, T. G. Mitchell, and J. Heitman
Chromosomal Translocation and Segmental Duplication in Cryptococcus neoformans
Eukaryot. Cell, February 1, 2005; 4(2): 401 - 406.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2004 by the Genetics Society of America.