Genetics, Vol. 167, 263-273, May 2004, Copyright © 2004

Mutational Analysis of the Drosophila DNA Repair and Recombination Gene mei-9

Özlem Yildiza, Hutton Kearneya, Benjamin C. Kramera, and Jeff J. Sekelskya,b
a Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599
b Program in Molecular Biology and Biotechnology, University of North Carolina, Chapel Hill, North Carolina 27599

Corresponding author: Jeff J. Sekelsky, 303 Fordham Hall, University of North Carolina, Chapel Hill, NC 27599., sekelsky{at}unc.edu (E-mail)

Communicating editor: K. G. GOLIC

Drosophila mei-9 is essential for several DNA repair and recombination pathways, including nucleotide excision repair (NER), interstrand crosslink repair, and meiotic recombination. To better understand the role of MEI-9 in these processes, we characterized 10 unique mutant alleles of mei-9. These include a P-element insertion that disrupts repair functions but not the meiotic function; three nonsense mutations, one of which has nearly wild-type levels of protein; three missense mutations, one of which disrupts the meiotic function but not repair functions; two small in-frame deletions; and one frameshift.




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