help button home button Genetics J Virology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Laurencon, A.
Right arrow Articles by Burtis, K. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Laurencon, A.
Right arrow Articles by Burtis, K. C.
Genetics, Vol. 167, 217-231, May 2004, Copyright © 2004

A Large-Scale Screen for Mutagen-Sensitive Loci in Drosophila

Anne Laurencona,b, Charisse M. Ormea,c, Heather K. Petersa,c, Christina L. Boultona,d, Eszter K. Vladara,e, Sasha A. Langleya, Emmanuel P. Bakisa, David T. Harrisa,c, Nathan J. Harrisa, Sarah M. Waysona, R. Scott Hawleya,c, and Kenneth C. Burtisa
a Section of Molecular and Cellular Biology, University of California, Davis, California 95616,
b Centre de Génétique Moléculaire et Cellulaire, Université Claude Bernard Lyon 1/UMR 5534, 69622 Villeurbanne Cedex, France,
c Stowers Institute for Medical Research, Kansas City, Missouri 64110,
d Howard Hughes Medical Institute, Harvard Institutes of Medicine, Boston, Massachusetts 02115
e Department of Genetics, Stanford University School of Medicine, Palo Alto, California 94305

Corresponding author: Kenneth C. Burtis, 1 Shields Ave., University of California, Davis, CA 95616., kcburtis{at}ucdavis.edu (E-mail)

Communicating editor: K. G. GOLIC

In a screen for new DNA repair mutants, we tested 6275 Drosophila strains bearing homozygous mutagenized autosomes (obtained from C. Zuker) for hypersensitivity to methyl methanesulfonate (MMS) and nitrogen mustard (HN2). Testing of 2585 second-chromosome lines resulted in the recovery of 18 mutants, 8 of which were alleles of known genes. The remaining 10 second-chromosome mutants were solely sensitive to MMS and define 8 new mutagen-sensitive genes (mus212–mus219). Testing of 3690 third chromosomes led to the identification of 60 third-chromosome mutants, 44 of which were alleles of known genes. The remaining 16 mutants define 14 new mutagen-sensitive genes (mus314–mus327). We have initiated efforts to identify these genes at the molecular level and report here the first two identified. The HN2-sensitive mus322 mutant defines the Drosophila ortholog of the yeast snm1 gene, and the MMS- and HN2-sensitive mus301 mutant defines the Drosophila ortholog of the human HEL308 gene. We have also identified a second-chromosome mutant, mus215ZIII-2059, that uniformly reduces the frequency of meiotic recombination to <3% of that observed in wild type and thus defines a function required for both DNA repair and meiotic recombination. At least one allele of each new gene identified in this study is available at the Bloomington Stock Center.




This article has been cited by other articles:


Home page
 GeneticsHome page
D. S. Wei and Y. S. Rong
A Genetic Screen For DNA Double-Strand Break Repair Mutations in Drosophila
Genetics, September 1, 2007; 177(1): 63 - 77.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
U. Abdu, M. Klovstad, V. Butin-Israeli, A. Bakhrat, and T. Schupbach
An essential role for Drosophila hus1 in somatic and meiotic DNA damage responses
J. Cell Sci., March 15, 2007; 120(6): 1042 - 1049.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
R. McCaffrey, D. St Johnston, and A. Gonzalez-Reyes
Drosophila mus301/spindle-C Encodes a Helicase With an Essential Role in Double-Strand DNA Break Repair and Meiotic Progression
Genetics, November 1, 2006; 174(3): 1273 - 1285.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
C. P. Guy and E. L. Bolt
Archaeal Hel308 helicase targets replication forks in vivo and in vitro and unwinds lagging strands
Nucleic Acids Res., June 30, 2005; 33(11): 3678 - 3690.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
N. Shima, R. J. Munroe, and J. C. Schimenti
The Mouse Genomic Instability Mutation chaos1 Is an Allele of Polq That Exhibits Genetic Interaction with Atm
Mol. Cell. Biol., December 1, 2004; 24(23): 10381 - 10389.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
E. J. Koundakjian, D. M. Cowan, R. W. Hardy, and A. H. Becker
The Zuker Collection: A Resource for the Analysis of Autosomal Gene Function in Drosophila melanogaster
Genetics, May 1, 2004; 167(1): 203 - 206.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
B. T. Wakimoto, D. L. Lindsley, and C. Herrera
Toward a Comprehensive Genetic Analysis of Male Fertility in Drosophila melanogaster
Genetics, May 1, 2004; 167(1): 207 - 216.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2004 by the Genetics Society of America.