help button home button Genetics J Gen Phys
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Srivastava, A.
Right arrow Articles by Bell, J. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Srivastava, A.
Right arrow Articles by Bell, J. B.
Genetics, Vol. 166, 1833-1843, April 2004, Copyright © 2004

Molecular and Functional Analysis of scalloped Recessive Lethal Alleles in Drosophila melanogaster

Ajay Srivastavaa, Andrew J. Simmondsb, Ankush Garga, Leif Fossheimb, Shelagh D. Campbella, and John B. Bella
a Department of Biological Sciences, University of Alberta, Edmonton, Alberta T6G 2E9, Canada
b Department of Cell Biology, University of Alberta, Edmonton, Alberta T6G 2E9, Canada

Corresponding author: John B. Bell, University of Alberta, Edmonton, AB T6G 2E9, Canada., jbell{at}ualberta.ca (E-mail)

Communicating editor: T. SCHÜPBACH

The Drosophila melanogaster scalloped (sd) gene is a homolog of the human TEF-1 gene and is a member of the TEA/ATTS domain-containing family of transcription factors. In Drosophila, sd is involved in wing development as well as neural development. Herein, data are presented from a molecular analysis of five recessive lethal sd alleles. Only one of these alleles complements a viable allele associated with an sd mutant wing phenotype, suggesting that functions important for wing development are compromised by the noncomplementing alleles. Two of the wing noncomplementing alleles have mutations that help to define a VG-binding domain for the SD protein in vivo, and another noncomplementing allele has a lesion within the TEA DNA-binding domain. The VG-binding domain overlaps with a domain important for viability of the fly, since two of the sd lethal lesions are located there. The fifth lethal affects a yet undefined motif lying just outside the VG-binding domain in the C-terminal direction that affects both wing phenotype and viability. This is the first example linking mutations affecting specific amino acids in the SD protein with phenotypic consequences for the organism.




This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
A. Sawada, H. Kiyonari, K. Ukita, N. Nishioka, Y. Imuta, and H. Sasaki
Redundant Roles of Tead1 and Tead2 in Notochord Development and the Regulation of Cell Proliferation and Survival
Mol. Cell. Biol., May 15, 2008; 28(10): 3177 - 3189.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
A. Garg, A. Srivastava, M. M. Davis, S. L. O'Keefe, L. Chow, and J. B. Bell
Antagonizing Scalloped With a Novel Vestigial Construct Reveals an Important Role for Scalloped in Drosophila melanogaster Leg, Eye and Optic Lobe Development
Genetics, February 1, 2007; 175(2): 659 - 669.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Anbanandam, D. C. Albarado, C. T. Nguyen, G. Halder, X. Gao, and S. Veeraraghavan
Insights into transcription enhancer factor 1 (TEF-1) activity from the solution structure of the TEA domain
PNAS, November 14, 2006; 103(46): 17225 - 17230.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2004 by the Genetics Society of America.