- THIS ARTICLE
- Full Text
- Full Text (PDF)
- Supplemental Table S1
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Email this article to a friend
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Krzywinski, J.
- Articles by Besansky, N. J.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Krzywinski, J.
- Articles by Besansky, N. J.
Isolation and Characterization of Y Chromosome Sequences From the African Malaria Mosquito Anopheles gambiae
Jaroslaw Krzywinskia, Deborah R. Nusskernb, Marcia K. Kerna, and Nora J. Besanskyaa Center for Tropical Disease Research and Training, Department of Biological Sciences, University of Notre Dame, Notre Dame, Indiana 46556
b Celera Genomics, Rockville, Maryland 20850
Corresponding author: Nora J. Besansky, University of Notre Dame, 317 Galvin Life Science Center, Notre Dame, IN 46556., nbesansk{at}nd.edu (E-mail)
Communicating editor: M. A. F. NOOR
10% of the genome, remains virtually unexplored, despite the recent completion of the A. gambiae genome project. Here we report the identification and characterization of Y chromosome sequences of total length approaching 150 kb. We developed 11 Y-specific PCR markers that consistently yielded male-specific products in specimens from both laboratory colony and natural populations. The markers are characterized by low sequence polymorphism in samples collected across Africa and by presence in more than one copy on the Y. Screening of the A. gambiae BAC library using these markers allowed detection of 90 Y-linked BAC clones. Analysis of the BAC sequences and other Y-derived fragments showed massive accumulation of a few transposable elements. Nevertheless, more complex sequences are apparently present on the Y; these include portions of an 48-kb-long unmapped AAAB01008227 scaffold from the whole genome shotgun assembly. Anopheles Y appears not to harbor any of the genes identified in Drosophila Y. However, experiments suggest that one of the ORFs from the AAAB01008227 scaffold represents a fragment of a gene with male-specific expression.
This article has been cited by other articles:
 |
|
 |
|
  K. R. Ng'habi, A. Horton, B. G. J. Knols, and G. C. Lanzaro A New Robust Diagnostic Polymerase Chain Reaction for Determining the Mating Status of Female Anopheles gambiae Mosquitoes Am J Trop Med Hyg, September 1, 2007; 77(3): 485 - 487. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Krzywinski, D. Sangare, and N. J. Besansky Satellite DNA From the Y Chromosome of the Malaria Vector Anopheles gambiae Genetics, January 1, 2005; 169(1): 185 - 196. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. C. Tubio, H. Naveira, and J. Costas Structural and Evolutionary Analyses of the Ty3/gypsy Group of LTR Retrotransposons in the Genome of Anopheles gambiae Mol. Biol. Evol., January 1, 2005; 22(1): 29 - 39. [Abstract] [Full Text] [PDF]
|
|