One-Hybrid Screens at the Saccharomyces cerevisiae HMR Locus Identify Novel Transcriptional Silencing Factors

doi:10.1534/genetics.166.1.631

One-Hybrid Screens at the Saccharomyces cerevisiae HMR Locus Identify Novel Transcriptional Silencing Factors

Erik D. Andrulis^a, David C. Zappulla^a, Krassimira Alexieva-Botcheva^a, Carlos Evangelista^b, and Rolf Sternglanz^a
^a Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, New York 11794
^b Department of Genome Sciences, University of Washington, Seattle, Washington 98195

Corresponding author: Rolf Sternglanz, Stony Brook University, Stony Brook, NY 11794-5215., rolf{at}life.bio.sunysb.edu (E-mail)

Communicating editor: F. WINSTON

In Saccharomyces cerevisiae, genes located at the telomeresand the HM loci are subject to transcriptional silencing. Here,we report results of screening a Gal4 DNA-binding domain hybridlibrary for proteins that cause silencing when targeted to asilencer-defective HMR locus.

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