Recombination Within a Nucleotide-Binding-Site/Leucine-Rich-Repeat Gene Cluster Produces New Variants Conditioning Resistance to Soybean Mosaic Virus in Soybeans

Corresponding author: M. A. Saghai Maroof, 328 Smyth Hall, Virginia Tech, Blacksburg, VA 24061-0404., smaroof{at}vt.edu (E-mail)

Communicating editor: A. H. PATERSON

The soybean Rsv1 gene for resistance to soybean mosaic virus (SMV; Potyvirus) has previously been described as a single-locus multi-allelic gene mapping to molecular linkage group (MLG) F. Various Rsv1 alleles condition different responses to the seven (G1–G7) described strains of SMV, including extreme resistance, localized and systemic necrosis, and mosaic symptoms. We describe the cloning of a cluster of NBS-LRR resistance gene candidates from MLG F of the virus-resistant soybean line PI96983 and demonstrate that multiple genes within this cluster interact to condition unique responses to SMV strains. In addition to cloning 3gG2, a strong candidate for the major Rsv1 resistance gene from PI96983, we describe various unique resistant and necrotic reactions coincident with the presence or absence of other members of this gene cluster. Responses of recombinant lines from a high-resolution mapping population of PI96983 (resistant) x Lee 68 (susceptible) demonstrate that more than one gene in this region of the PI96983 chromosome conditions resistance and/or necrosis to SMV. In addition, the soybean cultivars Marshall and Ogden, which carry other previously described Rsv1 alleles, are shown to possess the 3gG2 gene in a NBS-LRR gene cluster background distinct from PI96983. These observations suggest that two or more related non-TIR-NBS-LRR gene products are likely involved in the allelic response of several Rsv1-containing lines to SMV.

This article has been cited by other articles:

				D. M. Tucker, M. A. S. Maroof, S. C. Jeong, G. R. Buss, and S. A. Tolin Validation and Interaction of the Soybean Mosaic Virus Lethal Necrosis Allele, Rsv1-n, in PI 507389 Crop Sci., June 26, 2009; 49(4): 1277 - 1283. [Abstract] [Full Text] [PDF]

				V. Geffroy, C. Macadre, P. David, A. Pedrosa-Harand, M. Sevignac, C. Dauga, and T. Langin Molecular Analysis of a Large Subtelomeric Nucleotide-Binding-Site-Leucine-Rich-Repeat Family in Two Representative Genotypes of the Major Gene Pools of Phaseolus vulgaris Genetics, February 1, 2009; 181(2): 405 - 419. [Abstract] [Full Text] [PDF]

				R. W. Innes, C. Ameline-Torregrosa, T. Ashfield, E. Cannon, S. B. Cannon, B. Chacko, N. W.G. Chen, A. Couloux, A. Dalwani, R. Denny, et al. Differential Accumulation of Retroelements and Diversification of NB-LRR Disease Resistance Genes in Duplicated Regions following Polyploidy in the Ancestor of Soybean Plant Physiology, December 1, 2008; 148(4): 1740 - 1759. [Abstract] [Full Text] [PDF]

				M. L. Rosso, J. C. Rupe, P. Chen, and L. A. Mozzoni Inheritance and Genetic Mapping of Resistance to Pythium Damping-Off Caused by Pythium aphanidermatum in 'Archer' Soybean Crop Sci., November 24, 2008; 48(6): 2215 - 2222. [Abstract] [Full Text] [PDF]

				M. A. Saghai Maroof, S. C. Jeong, I. Gunduz, D. M. Tucker, G. R. Buss, and S. A. Tolin Pyramiding of Soybean Mosaic Virus Resistance Genes by Marker-Assisted Selection Crop Sci., March 19, 2008; 48(2): 517 - 526. [Abstract] [Full Text] [PDF]

				A. Shi, P. Chen, C. Zheng, A. Hou, and B. Zhang A PCR-based Marker for the Rsv1 Locus Conferring Resistance to Soybean Mosaic Virus Crop Sci., January 16, 2008; 48(1): 262 - 268. [Abstract] [Full Text] [PDF]

				Y. Wang, H. A. Hobbs, C. R. Bowen, R. L. Bernard, C. B. Hill, J. S. Haudenshield, L. L. Domier, and G. L. Hartman Evaluation of Soybean Cultivars, 'Williams' Isogenic Lines, and Other Selected Soybean Lines for Resistance to Two Soybean Mosaic Virus Strains Crop Sci., November 21, 2006; 46(6): 2649 - 2653. [Abstract] [Full Text] [PDF]

				J. Fletcher, C. Bender, B. Budowle, W. T. Cobb, S. E. Gold, C. A. Ishimaru, D. Luster, U. Melcher, R. Murch, H. Scherm, et al. Plant Pathogen Forensics: Capabilities, Needs, and Recommendations Microbiol. Mol. Biol. Rev., June 1, 2006; 70(2): 450 - 471. [Abstract] [Full Text] [PDF]

				S. G. Gordon, S. K. St. Martin, and A. E. Dorrance Rps8 Maps to a Resistance Gene Rich Region on Soybean Molecular Linkage Group F Crop Sci., December 2, 2005; 46(1): 168 - 173. [Abstract] [Full Text] [PDF]

				D. Sandhu, K. G. Schallock, N. Rivera-Velez, P. Lundeen, S. Cianzio, and M. K. Bhattacharyya Soybean Phytophthora Resistance Gene Rps8 Maps Closely to the Rps3 Region J. Hered., September 1, 2005; 96(5): 536 - 541. [Abstract] [Full Text] [PDF]

				D. Sandhu, H. Gao, S. Cianzio, and M. K. Bhattacharyya Deletion of a Disease Resistance Nucleotide-Binding-Site Leucine-Rich- Repeat-like Sequence Is Associated With the Loss of the Phytophthora Resistance Gene Rps4 in Soybean Genetics, December 1, 2004; 168(4): 2157 - 2167. [Abstract] [Full Text] [PDF]

				S. M. Smith, A. J. Pryor, and S. H. Hulbert Allelic and Haplotypic Diversity at the Rp1 Rust Resistance Locus of Maize Genetics, August 1, 2004; 167(4): 1939 - 1947. [Abstract] [Full Text] [PDF]