sel-7, a Positive Regulator of lin-12 Activity, Encodes a Novel Nuclear Protein in Caenorhabditis elegans

Corresponding author: Iva Greenwald, 701 W. 168th St., Room 720, College of Physicians and Surgeons, Columbia University, New York, NY 10032., greenwald{at}cancercenter.columbia.edu (E-mail)

Communicating editor: B. J. MEYER

Suppressor genetics in C. elegans has identified key components of the LIN-12/Notch signaling pathway. Here, we describe a genetic and molecular characterization of the suppressor gene sel-7. We show that reducing or eliminating sel-7 activity suppresses the effects of constitutive lin-12 activity, enhances the effects of partially reduced lin-12 activity, and causes a synthetic Lin-12(0) phenotype when combined with a null mutation in the sel-12 presenilin gene. These observations suggest that sel-7 is a positive regulator of lin-12 activity. We also show that SEL-7 encodes a novel nuclear protein. Through yeast two-hybrid screening, we identified an apparent interaction partner, K08E3.8, that also interacts with SEL-8, a known component of the nuclear complex that forms upon LIN-12 activation. Our data suggest potential roles for SEL-7 in the assembly or function of this nuclear complex.

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