| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
q Regulates Egg-Laying Behavior via a PLCß-Independent and Serotonin-Dependent Signaling Pathway and Likely Functions Both in the Nervous System and in Muscle
Corresponding author: Paul W. Sternberg, California Institute of Technology, Pasadena, CA 91125., pws{at}its.caltech.edu (E-mail)
Communicating editor: B. J. MEYER
q family members. We analyzed the expression pattern of EGL-30 and found that it is broadly expressed, with highest expression in the nervous system and in pharyngeal muscle. We isolated dominant, gain-of-function alleles of egl-30 as intragenic revertants of an egl-30 reduction-of-function mutation. Using these gain-of-function mutants and existing reduction-of-function mutants, we examined the site and mode of action of EGL-30. On the basis of pharmacological analysis, it has been determined that egl-30 functions both in the nervous system and in the vulval muscles for egg-laying behavior. Genetic epistasis over mutations that eliminate detectable levels of serotonin reveals that egl-30 requires serotonin to regulate egg laying. Furthermore, pharmacological response assays strongly suggest that EGL-30 may directly couple to a serotonin receptor to mediate egg laying. We also examined genetic interactions with mutations in the gene that encodes the single C. elegans homolog of PLCß and mutations in genes that encode signaling molecules downstream of PLCß. We conclude that PLCß functions in parallel with egl-30 with respect to egg laying or is not the major effector of EGL-30. In contrast, PLCß-mediated signaling is likely downstream of EGL-30 with respect to pharyngeal-pumping behavior. Our data indicate that there are multiple signaling pathways downstream of EGL-30 and that different pathways could predominate with respect to the regulation of different behaviors.
This article has been cited by other articles:
 |
|
 |
|
  H.-C. Lee, T. Inoue, R. Imae, N. Kono, S. Shirae, S. Matsuda, K. Gengyo-Ando, S. Mitani, and H. Arai Caenorhabditis elegans mboa-7, a Member of the MBOAT Family, Is Required for Selective Incorporation of Polyunsaturated Fatty Acids into Phosphatidylinositol Mol. Biol. Cell, March 1, 2008; 19(3): 1174 - 1184. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. E. Tanis, J. J. Moresco, R. A. Lindquist, and M. R. Koelle Regulation of Serotonin Biosynthesis by the G Proteins G{alpha}o and G{alpha}q Controls Serotonin Signaling in Caenorhabditis elegans Genetics, January 1, 2008; 178(1): 157 - 169. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. L. Williams, S. Lutz, N. K. Charlie, C. Vettel, M. Ailion, C. Coco, J. J.G. Tesmer, E. M. Jorgensen, T. Wieland, and K. G. Miller Trio's Rho-specific GEF domain is the missing G{alpha}q effector in C. elegans Genes & Dev., November 1, 2007; 21(21): 2731 - 2746. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Liu, B. LeBoeuf, and L. R. Garcia G{alpha}q-Coupled Muscarinic Acetylcholine Receptors Enhance Nicotinic Acetylcholine Receptor Signaling in Caenorhabditis elegans Mating Behavior J. Neurosci., February 7, 2007; 27(6): 1411 - 1421. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Yook and J. Hodgkin Mos1 Mutagenesis Reveals a Diversity of Mechanisms Affecting Response of Caenorhabditis elegans to the Bacterial Pathogen Microbacterium nematophilum Genetics, February 1, 2007; 175(2): 681 - 697. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Suo, Y. Kimura, and H. H. M. Van Tol Starvation Induces cAMP Response Element-Binding Protein-Dependent Gene Expression through Octopamine-Gq Signaling in Caenorhabditis elegans J. Neurosci., October 4, 2006; 26(40): 10082 - 10090. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Clejan, J. Boerckel, and S. Ahmed Developmental Modulation of Nonhomologous End Joining in Caenorhabditis elegans Genetics, July 1, 2006; 173(3): 1301 - 1317. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Chen, E. M. Talley, N. Patel, A. Gomis, W. E. McIntire, B. Dong, F. Viana, J. C. Garrison, and D. A. Bayliss Inhibition of a background potassium channel by Gq protein {alpha}-subunits PNAS, February 28, 2006; 103(9): 3422 - 3427. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Carnell, J. Illi, S. W. Hong, and S. L. McIntire The G-Protein-Coupled Serotonin Receptor SER-1 Regulates Egg Laying and Male Mating Behaviors in Caenorhabditis elegans J. Neurosci., November 16, 2005; 25(46): 10671 - 10681. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. J. D. Gower, D. S. Walker, and H. A. Baylis Inositol 1,4,5-Trisphosphate Signaling Regulates Mating Behavior in Caenorhabditis elegans Males Mol. Biol. Cell, September 1, 2005; 16(9): 3978 - 3986. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Dempsey, S. M. Mackenzie, A. Gargus, G. Blanco, and J. Y. Sze Serotonin (5HT), Fluoxetine, Imipramine and Dopamine Target Distinct 5HT Receptor Signaling to Modulate Caenorhabditis elegans Egg-Laying Behavior Genetics, March 1, 2005; 169(3): 1425 - 1436. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Schade, N. K. Reynolds, C. M. Dollins, and K. G. Miller Mutations That Rescue the Paralysis of Caenorhabditis elegans ric-8 (Synembryn) Mutants Activate the G{alpha}s Pathway and Define a Third Major Branch of the Synaptic Signaling Network Genetics, February 1, 2005; 169(2): 631 - 649. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. M. Hajdu-Cronin, W. J. Chen, and P. W. Sternberg The L-Type Cyclin CYL-1 and the Heat-Shock-Factor HSF-1 Are Required for Heat-Shock-Induced Protein Expression in Caenorhabditis elegans Genetics, December 1, 2004; 168(4): 1937 - 1949. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Y. Chao, H. Komatsu, H. S. Fukuto, H. M. Dionne, and A. C. Hart Feeding status and serotonin rapidly and reversibly modulate a Caenorhabditis elegans chemosensory circuit PNAS, October 26, 2004; 101(43): 15512 - 15517. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
