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Genetics, Vol. 165, 1703-1715, December 2003, Copyright © 2003

The N-Terminal DNA-Binding Domain of Rad52 Promotes RAD51-Independent Recombination in Saccharomyces cerevisiae

Mariko Tsukamotoa, Kentaro Yamashitaa, Toshiko Miyazakia, Miki Shinoharaa, and Akira Shinoharaa,b
a Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan
b Japanese Science and Technology (JST), Toyonaka, Osaka 560-0043, Japan

Corresponding author: Akira Shinohara, Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 560-0043, Japan., ashino{at}bio.sci.osaka-u.ac.jp (E-mail)

Communicating editor: L. SYMINGTON

In Saccharomyces cerevisiae, the Rad52 protein plays a role in both RAD51-dependent and RAD51-independent recombination pathways. We characterized a rad52 mutant, rad52-329, which lacks the C-terminal Rad51-interacting domain, and studied its role in RAD51-independent recombination. The rad52-329 mutant is completely defective in mating-type switching, but partially proficient in recombination between inverted repeats. We also analyzed the effect of the rad52-329 mutant on telomere recombination. Yeast cells lacking telomerase maintain telomere length by recombination. The rad52-329 mutant is deficient in RAD51-dependent telomere recombination, but is proficient in RAD51-independent telomere recombination. In addition, we examined the roles of other recombination genes in the telomere recombination. The RAD51-independent recombination in the rad52-329 mutant is promoted by a paralogue of Rad52, Rad59. All components of the Rad50-Mre11-Xrs2 complex are also important, but not essential, for RAD51-independent telomere recombination. Interestingly, RAD51 inhibits the RAD51-independent, RAD52-dependent telomere recombination. These findings indicate that Rad52 itself, and more precisely its N-terminal DNA-binding domain, promote an essential reaction in recombination in the absence of RAD51.




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