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Genetics, Vol. 165, 835-848, October 2003, Copyright © 2003

Duplication-Dependent CG Suppression of the Seed Storage Protein Genes of Maize

Gertrud Lunda, Massimiliano Lauriaa, Per Guldbergb, and Silvio Zainac
a Plant Biochemistry Laboratory, Department of Plant Biology, The Royal Veterinary and Agricultural University, DK-1871 Frederiksberg C, Denmark,
b Institute of Cancer Biology, Danish Cancer Society, DK-2100 Copenhagen, Denmark
c Experimental Cardiovascular Research, Wallenberg Laboratory, Department of Medicine, University of Lund, 205 02 Malmø, Sweden

Corresponding author: Gertrud Lund, Department of Plant Biology, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark., gel{at}kvl.dk (E-mail)

Communicating editor: J. BIRCHLER

This study investigates the prevalence of CG and CNG suppression in single- vs. multicopy DNA regions of the maize genome. The analysis includes the single- and multicopy seed storage proteins (zeins), the miniature inverted-repeat transposable elements (MITEs), and long terminal repeat (LTR) retrotransposons. Zein genes are clustered on specific chromosomal regions, whereas MITEs and LTRs are dispersed in the genome. The multicopy zein genes are CG suppressed and exhibit large variations in CG suppression. The variation observed correlates with the extent of duplication each zein gene has undergone, indicating that gene duplication results in an increased turnover of cytosine residues. Alignment of individual zein genes confirms this observation and demonstrates that CG depletion results primarily from polarized C:T and G:A transition mutations from a less to a more extensively duplicated gene. In addition, transition mutations occur primarily in a CG or CNG context suggesting that CG suppression may result from deamination of methylated cytosine residues. Duplication-dependent CG depletion is likely to occur at other loci as duplicated MITEs and LTR elements, or elements inserted into duplicated gene regions, also exhibit CG depletion.






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Copyright © 2003 by the Genetics Society of America.