An AFLP-Based Genetic Linkage Map of Channel Catfish (Ictalurus punctatus) Constructed by Using an Interspecific Hybrid Resource Family

An AFLP-Based Genetic Linkage Map of Channel Catfish (Ictalurus punctatus) Constructed by Using an Interspecific Hybrid Resource Family

Zhanjiang Liu^a, Attila Karsi^a, Ping Li^a, Dongfeng Cao^a, and R. Dunham^a
^a The Fish Molecular Genetics and Biotechnology Laboratory, Department of Fisheries and Allied Aquacultures Program of Cell and Molecular Biosciences, Auburn University, Auburn, Alabama 36849

Corresponding author: Zhanjiang Liu, Auburn University, Auburn, AL 36849., zliu{at}acesag.auburn.edu (E-mail)

Communicating editor: M. SIMMONS

Catfish is the major aquaculture species in the United States.The hybrid catfish produced by crossing channel catfish femaleswith blue catfish males exhibit a number of desirable productiontraits, but their mass production has been difficult. To introducedesirable genes from blue catfish into channel catfish throughintrogression, a genetic linkage map is helpful. In this project,a genetic linkage map was constructed using amplified fragmentlength polymorphism (AFLP). A total of 607 AFLP markers wereanalyzed using 65 primer combinations and an interspecific backcrossresource family. A total of 418 AFLP markers were assigned to44 linkage groups. Among the remaining 189 markers, 101 werenot used because of significant segregation distortion, 29 wereunlinked, and 59 were eliminated because they span very largedistances. The 418 AFLP markers covered 1593 cM Kosambi. TheAFLP markers showed a high level of clustering that appearsto be related to certain primer combinations. This linkage mapwill serve as the basis for mapping a greater number of markersto provide a map with high enough resolution for it to be usefulfor selective breeding programs using introgression.

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