The G1 Cyclin Cln3p Controls Vacuolar Biogenesis in Saccharomyces cerevisiae

The G₁ Cyclin Cln3p Controls Vacuolar Biogenesis in Saccharomyces cerevisiae

Bong-Kwan Han^a, Rodolfo Aramayo^b, and Michael Polymenis^a
^a Department of Biochemistry and Biophysics, Program in Microbial Genetics and Genomics, Texas A&M University, College Station, Texas 77843
^b Department of Biology, Program in Microbial Genetics and Genomics, Texas A&M University, College Station, Texas 77843

Corresponding author: Michael Polymenis, Texas A&M University, 2128 TAMU, College Station, TX 77843-2128., polymenis{at}tamu.edu (E-mail)

Communicating editor: M. SACHS

How organelle biogenesis and inheritance is linked to cell divisionis poorly understood. In the budding yeast Saccharomyces cerevisiaethe G₁ cyclins Cln1,2,3p control initiation of cell division.Here we show that Cln3p controls vacuolar (lysosomal) biogenesisand segregation. First, loss of Cln3p, but not Cln1p or Cln2p,resulted in vacuolar fragmentation. Although the vacuoles ofcln3 ${Delta}$ cells were fragmented, together they occupied a large space,which accounted for a significant fraction of the overall cellsize increase in cln3 ${Delta}$ cells. Second, cytosol prepared from cellslacking Cln3p had reduced vacuolar homotypic fusion activityin cell-free assays. Third, vacuolar segregation was perturbedin cln3 ${Delta}$ cells. Our findings reveal a novel role for a eukaryoticG₁ cyclin in cytoplasmic organelle biogenesis and segregation.

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