Genetics, Vol. 165, 127-144, September 2003, Copyright © 2003

Isolation and Characterization of High-Temperature-Induced Dauer Formation Mutants in Caenorhabditis elegans

Michael Ailiona and James H. Thomasa,b
a Molecular and Cellular Biology Program of the University of Washington and Fred Hutchinson Cancer Research Center, University of Washington, Seattle, Washington 98195
b Department of Genome Sciences, University of Washington, Seattle, Washington 98195

Corresponding author: James H. Thomas, University of Washington, Box 357730, Seattle, WA 98195., jht{at}u.washington.edu (E-mail)

Communicating editor: P. ANDERSON

Dauer formation in Caenorhabditis elegans is regulated by at least three signaling pathways, including an insulin receptor-signaling pathway. These pathways were defined by mutants that form dauers constitutively (Daf-c) at 25°. Screens for Daf-c mutants at 25° have probably been saturated, but failed to identify all the components involved in regulating dauer formation. Here we screen for Daf-c mutants at 27°, a more strongly dauer-inducing condition. Mutations identified include novel classes of alleles for three known genes and alleles defining at least seven new genes, hid-1–hid-7. Many of the genes appear to act in the insulin branch of the dauer pathway, including pdk-1, akt-1, aex-6, and hid-1. We also molecularly identify hid-1 and show that it encodes a novel highly conserved putative transmembrane protein expressed in neurons.




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