- THIS ARTICLE
- Full Text
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Email this article to a friend
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Manning, C. M.
- Articles by Thackeray, J. R.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Manning, C. M.
- Articles by Thackeray, J. R.
Phospholipase C-
Contains Introns Shared by src Homology 2 Domains in Many Unrelated Proteins
Charlene M. Manninga,
Wendy R. Mathewsa,
Leah P. Ficoa, and
Justin R. Thackeraya
a Biology Department, Clark University, Worcester, Massachusetts 01610
Corresponding author: Justin R. Thackeray, Clark University, 950 Main St., Worcester, MA 01610., jthackeray{at}clarku.edu (E-mail)
Communicating editor: J. HEY
(PLC-) is typical of proteins that appeared at this time, containing several different modules that probably originated elsewhere. To gain insight into both PLC- evolution and structure-function relationships within the Drosophila PLC- encoded by small wing (sl), we cloned and sequenced the PLC- homologs from Drosophila pseudoobscura and D. virilis and compared their gene structure and predicted amino acid sequences with PLC- homologs in other animals. PLC- has been well conserved throughout, although structural differences suggest that the role of tyrosine phosphorylation in enzyme activation differs between vertebrates and invertebrates. Comparison of intron positions demonstrates that extensive intron loss has occurred during invertebrate evolution and also reveals the presence of conserved introns in both the N- and C-terminal PLC- SH2 domains that are present in SH2 domains in many other genes. These and other conserved SH2 introns suggest that the SH2 domains in PLC- are derived from an ancestral domain that was shuffled not only into PLC-, but also into many other unrelated genes during animal evolution.
This article has been cited by other articles:
 |
|
 |
|
  S. Banerjee, R. Joshi, G. Venkiteswaran, N. Agrawal, S. Srikanth, F. Alam, and G. Hasan Compensation of inositol 1,4,5-trisphosphate receptor function by altering sarco-endoplasmic reticulum calcium ATPase activity in the Drosophila flight circuit. J. Neurosci., August 9, 2006; 26(32): 8278 - 8288. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Mankidy, J. Hastings, and J. R. Thackeray Distinct Phospholipase C-{gamma}-Dependent Signaling Pathways in the Drosophila Eye and Wing Are Revealed by a New small wing Allele Genetics, June 1, 2003; 164(2): 553 - 563. [Abstract] [Full Text] [PDF]
|
|