Genetics, Vol. 163, 1221-1225, March 2003, Copyright © 2003

Chromatin Immunoprecipitation Reveals That the 180-bp Satellite Repeat Is the Key Functional DNA Element of Arabidopsis thaliana Centromeres

Kiyotaka Nagakia, Paul B. Talbertb, Cathy Xiaoyan Zhongc, R. Kelly Dawec, Steven Henikoffb, and Jiming Jianga
a Department of Horticulture, University of Wisconsin, Madison, Wisconsin 53706,
b Howard Hughes Medical Institute, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109-1024
c Department of Plant Biology and Department of Genetics, University of Georgia, Athens, Georgia 30602

Corresponding author: Jiming Jiang, 1575 Linden Dr., University of Wisconsin, Madison, WI 53706., jjiang1{at}facstaff.wisc.edu (E-mail)

Communicating editor: V. L. CHANDLER

The centromeres of Arabidopsis thaliana chromosomes contain megabases of complex DNA consisting of numerous types of repetitive DNA elements. We developed a chromatin immunoprecipitation (ChIP) technique using an antibody against the centromeric H3 histone, HTR12, in Arabidopsis. ChIP assays showed that the 180-bp centromeric satellite repeat was precipitated with the antibody, suggesting that this repeat is the key component of the centromere/kinetochore complex in Arabidopsis.




This article has been cited by other articles:


Home page
 Plant Cell PhysiolHome page
L. Guo, S. Chen, K. Liu, Y. Liu, L. Ni, K. Zhang, and L. Zhang
Isolation of Heat Shock Factor HsfA1a-Binding Sites in vivo Revealed Variations of Heat Shock Elements in Arabidopsis thaliana
Plant Cell Physiol., September 1, 2008; 49(9): 1306 - 1315.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Murata, E. Yokota, F. Shibata, and K. Kashihara
Functional analysis of the Arabidopsis centromere by T-DNA insertion-induced centromere breakage
PNAS, May 27, 2008; 105(21): 7511 - 7516.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
R. Komiya, A. Ikegami, S. Tamaki, S. Yokoi, and K. Shimamoto
Hd3a and RFT1 are essential for flowering in rice
Development, February 15, 2008; 135(4): 767 - 774.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
W. Zhang, H.-R. Lee, D.-H. Koo, and J. Jiang
Epigenetic Modification of Centromeric Chromatin: Hypomethylation of DNA Sequences in the CENH3-Associated Chromatin in Arabidopsis thaliana and Maize
PLANT CELL, January 1, 2008; 20(1): 25 - 34.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
R. Rajagopalan, H. Vaucheret, J. Trejo, and D. P. Bartel
A diverse and evolutionarily fluid set of microRNAs in Arabidopsis thaliana
Genes & Dev., December 15, 2006; 20(24): 3407 - 3425.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
A. Kawabe, S. Nasuda, and D. Charlesworth
Duplication of Centromeric Histone H3 (HTR12) Gene in Arabidopsis halleri and A. lyrata, Plant Species With Multiple Centromeric Satellite Sequences
Genetics, December 1, 2006; 174(4): 2021 - 2032.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
I. Lermontova, V. Schubert, J. Fuchs, S. Klatte, J. Macas, and I. Schubert
Loading of Arabidopsis Centromeric Histone CENH3 Occurs Mainly during G2 and Requires the Presence of the Histone Fold Domain
PLANT CELL, October 1, 2006; 18(10): 2443 - 2451.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
H. Yan, H. Ito, K. Nobuta, S. Ouyang, W. Jin, S. Tian, C. Lu, R.C. Venu, G.-l. Wang, P. J. Green, et al.
Genomic and Genetic Characterization of Rice Cen3 Reveals Extensive Transcription and Evolutionary Implications of a Complex Centromere
PLANT CELL, September 1, 2006; 18(9): 2123 - 2133.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
R. M. Stupar, K. A. Beaubien, W. Jin, J. Song, M.-K. Lee, C. Wu, H.-B. Zhang, B. Han, and J. Jiang
Structural Diversity and Differential Transcription of the Patatin Multicopy Gene Family During Potato Tuber Development
Genetics, February 1, 2006; 172(2): 1263 - 1275.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
R. T. GRANT-DOWNTON and H. G. DICKINSON
Epigenetics and its Implications for Plant Biology. 1. The Epigenetic Network in Plants
Ann. Bot., December 1, 2005; 96(7): 1143 - 1164.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
S. E. Hall, S. Luo, A. E. Hall, and D. Preuss
Differential Rates of Local and Global Homogenization in Centromere Satellites From Arabidopsis Relatives
Genetics, August 1, 2005; 170(4): 1913 - 1927.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
K. Nagaki, K. Kashihara, and M. Murata
Visualization of Diffuse Centromeres with Centromere-Specific Histone H3 in the Holocentric Plant Luzula nivea
PLANT CELL, July 1, 2005; 17(7): 1886 - 1893.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
J.-Y. Lin, B. H. Jacobus, P. SanMiguel, J. G. Walling, Y. Yuan, R. C. Shoemaker, N. D. Young, and S. A. Jackson
Pericentromeric Regions of Soybean (Glycine max L. Merr.) Chromosomes Consist of Retroelements and Tandemly Repeated DNA and Are Structurally and Evolutionarily Labile
Genetics, July 1, 2005; 170(3): 1221 - 1230.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
W. Jin, J. C. Lamb, J. M. Vega, R. K. Dawe, J. A. Birchler, and J. Jiang
Molecular and Functional Dissection of the Maize B Chromosome Centromere
PLANT CELL, May 1, 2005; 17(5): 1412 - 1423.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
N. S. Edwards and A. W. Murray
Identification of Xenopus CENP-A and an Associated Centromeric DNA Repeat
Mol. Biol. Cell, April 1, 2005; 16(4): 1800 - 1810.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
F. Shibata and M. Murata
Differential localization of the centromere-specific proteins in the major centromeric satellite of Arabidopsis thaliana
J. Cell Sci., June 15, 2004; 117(14): 2963 - 2970.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
Y. Zhang, Y. Huang, L. Zhang, Y. Li, T. Lu, Y. Lu, Q. Feng, Q. Zhao, Z. Cheng, Y. Xue, et al.
Structural features of the rice chromosome 4 centromere
Nucleic Acids Res., April 2, 2004; 32(6): 2023 - 2030.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
W. Jin, J. R. Melo, K. Nagaki, P. B. Talbert, S. Henikoff, R. K. Dawe, and J. Jiang
Maize Centromeres: Organization and Functional Adaptation in the Genetic Background of Oat
PLANT CELL, March 1, 2004; 16(3): 571 - 581.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
R. J. Mroczek and R. K. Dawe
Distribution of Retroelements in Centromeres and Neocentromeres of Maize
Genetics, October 1, 2003; 165(2): 809 - 819.
[Abstract] [Full Text] [PDF]