Large-Scale Functional Genomic Analysis of Sporulation and Meiosis in Saccharomyces cerevisiae

Corresponding author: William S. Saunders, Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260., wsaund{at}pitt.edu (E-mail)

Communicating editor: M. JOHNSTON

We have used a single-gene deletion mutant bank to identify the genes required for meiosis and sporulation among 4323 nonessential Saccharomyces cerevisiae annotated open reading frames (ORFs). Three hundred thirty-four sporulation-essential genes were identified, including 78 novel ORFs and 115 known genes without previously described sporulation defects in the comprehensive Saccharomyces Genome (SGD) or Yeast Proteome (YPD) phenotype databases. We have further divided the uncharacterized sporulation-essential genes into early, middle, and late stages of meiosis according to their requirement for IME1 induction and nuclear division. We believe this represents a nearly complete identification of the genes uniquely required for this complex cellular pathway. The set of genes identified in this phenotypic screen shows only limited overlap with those identified by expression-based studies.

This article has been cited by other articles:

				F. J. Vizeacoumar, N. van Dyk, F. S.Vizeacoumar, V. Cheung, J. Li, Y. Sydorskyy, N. Case, Z. Li, A. Datti, C. Nislow, et al. Integrating high-throughput genetic interaction mapping and high-content screening to explore yeast spindle morphogenesis J. Cell Biol., January 11, 2010; 188(1): 69 - 81. [Abstract] [Full Text] [PDF]

				M. Seifert, J. Keilwagen, M. Strickert, and I. Grosse Utilizing gene pair orientations for HMM-based analysis of promoter array ChIP-chip data Bioinformatics, August 15, 2009; 25(16): 2118 - 2125. [Abstract] [Full Text] [PDF]

				K. Peisker, D. Braun, T. Wolfle, J. Hentschel, U. Funfschilling, G. Fischer, A. Sickmann, and S. Rospert Ribosome-associated Complex Binds to Ribosomes in Close Proximity of Rpl31 at the Exit of the Polypeptide Tunnel in Yeast Mol. Biol. Cell, December 1, 2008; 19(12): 5279 - 5288. [Abstract] [Full Text] [PDF]

				S. Hausmann, S. Zheng, M. Costanzo, R. L. Brost, D. Garcin, C. Boone, S. Shuman, and B. Schwer Genetic and Biochemical Analysis of Yeast and Human Cap Trimethylguanosine Synthase: FUNCTIONAL OVERLAP OF 2,2,7-TRIMETHYLGUANOSINE CAPS, SMALL NUCLEAR RIBONUCLEOPROTEIN COMPONENTS, PRE-mRNA SPLICING FACTORS, AND RNA DECAY PATHWAYS J. Biol. Chem., November 14, 2008; 283(46): 31706 - 31718. [Abstract] [Full Text] [PDF]

				S. A. Dighe and K. G. Kozminski Swf1p, a Member of the DHHC-CRD Family of Palmitoyltransferases, Regulates the Actin Cytoskeleton and Polarized Secretion Independently of Its DHHC Motif Mol. Biol. Cell, October 1, 2008; 19(10): 4454 - 4468. [Abstract] [Full Text] [PDF]

				J. M. Kingsbury and J. H. McCusker Threonine biosynthetic genes are essential in Cryptococcus neoformans Microbiology, September 1, 2008; 154(9): 2767 - 2775. [Abstract] [Full Text] [PDF]

				A. Auger, L. Galarneau, M. Altaf, A. Nourani, Y. Doyon, R. T. Utley, D. Cronier, S. Allard, and J. Cote Eaf1 Is the Platform for NuA4 Molecular Assembly That Evolutionarily Links Chromatin Acetylation to ATP-Dependent Exchange of Histone H2A Variants Mol. Cell. Biol., April 1, 2008; 28(7): 2257 - 2270. [Abstract] [Full Text] [PDF]

				L. Lariviere, M. Seizl, S. van Wageningen, S. Rother, L. van de Pasch, H. Feldmann, K. Strasser, S. Hahn, F. C.P. Holstege, and P. Cramer Structure-system correlation identifies a gene regulatory Mediator submodule Genes & Dev., April 1, 2008; 22(7): 872 - 877. [Abstract] [Full Text] [PDF]

				T. Iwaki, H. Iefuji, Y. Hiraga, A. Hosomi, T. Morita, Y. Giga-Hama, and K. Takegawa Multiple functions of ergosterol in the fission yeast Schizosaccharomyces pombe Microbiology, March 1, 2008; 154(3): 830 - 841. [Abstract] [Full Text] [PDF]

				Y. Suda, H. Nakanishi, E. M. Mathieson, and A. M. Neiman Alternative Modes of Organellar Segregation during Sporulation in Saccharomyces cerevisiae Eukaryot. Cell, November 1, 2007; 6(11): 2009 - 2017. [Abstract] [Full Text] [PDF]

				B. L. Pike and J. Heierhorst Mdt1 Facilitates Efficient Repair of Blocked DNA Double-Strand Breaks and Recombinational Maintenance of Telomeres Mol. Cell. Biol., September 15, 2007; 27(18): 6532 - 6545. [Abstract] [Full Text] [PDF]

				H. Nakanishi, Y. Suda, and A. M. Neiman Erv14 family cargo receptors are necessary for ER exit during sporulation in Saccharomyces cerevisiae J. Cell Sci., March 1, 2007; 120(5): 908 - 916. [Abstract] [Full Text] [PDF]

				C. Nouet, M. Bourens, O. Hlavacek, S. Marsy, C. Lemaire, and G. Dujardin Rmd9p Controls the Processing/Stability of Mitochondrial mRNAs and Its Overexpression Compensates for a Partial Deficiency of Oxa1p in Saccharomyces cerevisiae Genetics, March 1, 2007; 175(3): 1105 - 1115. [Abstract] [Full Text] [PDF]

				E. H. Williams, C. A. Butler, N. Bonnefoy, and T. D. Fox Translation Initiation in Saccharomyces cerevisiae Mitochondria: Functional Interactions Among Mitochondrial Ribosomal Protein Rsm28p, Initiation Factor 2, Methionyl-tRNA-Formyltransferase and Novel Protein Rmd9p Genetics, March 1, 2007; 175(3): 1117 - 1126. [Abstract] [Full Text] [PDF]

				X. H. Hu, M. H. Wang, T. Tan, J. R. Li, H. Yang, L. Leach, R. M. Zhang, and Z. W. Luo Genetic Dissection of Ethanol Tolerance in the Budding Yeast Saccharomyces cerevisiae Genetics, March 1, 2007; 175(3): 1479 - 1487. [Abstract] [Full Text] [PDF]

				J. A. Hill and S. P. Otto The Role of Pleiotropy in the Maintenance of Sex in Yeast Genetics, March 1, 2007; 175(3): 1419 - 1427. [Abstract] [Full Text] [PDF]

				S. Komili and F. P. Roth Genetic interaction screens advance in reverse Genes & Dev., January 15, 2007; 21(2): 137 - 142. [Full Text] [PDF]

				A. Gattiker, C. Niederhauser-Wiederkehr, J. Moore, L. Hermida, and M. Primig The GermOnline cross-species systems browser provides comprehensive information on genes and gene products relevant for sexual reproduction Nucleic Acids Res., January 12, 2007; 35(suppl_1): D457 - D462. [Abstract] [Full Text] [PDF]

				J. Li, S. Agarwal, and G. S. Roeder SSP2 and OSW1, Two Sporulation-Specific Genes Involved in Spore Morphogenesis in Saccharomyces cerevisiae Genetics, January 1, 2007; 175(1): 143 - 154. [Abstract] [Full Text] [PDF]

				A. Gonzalez, A. Ruiz, R. Serrano, J. Arino, and A. Casamayor Transcriptional Profiling of the Protein Phosphatase 2C Family in Yeast Provides Insights into the Unique Functional Roles of Ptc1 J. Biol. Chem., November 17, 2006; 281(46): 35057 - 35069. [Abstract] [Full Text] [PDF]

				J. P. Gerke, C. T. L. Chen, and B. A. Cohen Natural Isolates of Saccharomyces cerevisiae Display Complex Genetic Variation in Sporulation Efficiency Genetics, October 1, 2006; 174(2): 985 - 997. [Abstract] [Full Text] [PDF]

				K. L. Auld, A. L. Hitchcock, H. K. Doherty, S. Frietze, L. S. Huang, and P. A. Silver The Conserved ATPase Get3/Arr4 Modulates the Activity of Membrane-Associated Proteins in Saccharomyces cerevisiae Genetics, September 1, 2006; 174(1): 215 - 227. [Abstract] [Full Text] [PDF]

				G. Lesage and H. Bussey Cell Wall Assembly in Saccharomyces cerevisiae Microbiol. Mol. Biol. Rev., June 1, 2006; 70(2): 317 - 343. [Abstract] [Full Text] [PDF]

				P. M. Kane The Where, When, and How of Organelle Acidification by the Yeast Vacuolar H+-ATPase Microbiol. Mol. Biol. Rev., March 1, 2006; 70(1): 177 - 191. [Abstract] [Full Text] [PDF]

				J. E. Connolly and J. Engebrecht The Arf-GTPase-Activating Protein Gcs1p Is Essential for Sporulation and Regulates the Phospholipase D Spo14p Eukaryot. Cell, January 1, 2006; 5(1): 112 - 124. [Abstract] [Full Text] [PDF]

				A. M. Neiman Ascospore Formation in the Yeast Saccharomyces cerevisiae Microbiol. Mol. Biol. Rev., December 1, 2005; 69(4): 565 - 584. [Abstract] [Full Text] [PDF]

				C. M. McDonald, K. F. Cooper, and E. Winter The Ama1-Directed Anaphase-Promoting Complex Regulates the Smk1 Mitogen-Activated Protein Kinase During Meiosis in Yeast Genetics, November 1, 2005; 171(3): 901 - 911. [Abstract] [Full Text] [PDF]

				M. Sambade, M. Alba, A. M. Smardon, R. W. West, and P. M. Kane A Genomic Screen for Yeast Vacuolar Membrane ATPase Mutants Genetics, August 1, 2005; 170(4): 1539 - 1551. [Abstract] [Full Text] [PDF]

				M. Morishita and J. Engebrecht End3p-Mediated Endocytosis Is Required for Spore Wall Formation in Saccharomyces cerevisiae Genetics, August 1, 2005; 170(4): 1561 - 1574. [Abstract] [Full Text] [PDF]

				M. Y. Galperin and E. V. Koonin 'Conserved hypothetical' proteins: prioritization of targets for experimental study Nucleic Acids Res., October 12, 2004; 32(18): 5452 - 5463. [Abstract] [Full Text] [PDF]

				F. Gomez-Esquer, J. M. Rodriguez-Pena, G. Diaz, E. Rodriguez, P. Briza, C. Nombela, and J. Arroyo CRR1, a gene encoding a putative transglycosidase, is required for proper spore wall assembly in Saccharomyces cerevisiae Microbiology, October 1, 2004; 150(10): 3269 - 3280. [Abstract] [Full Text] [PDF]

				K. Yamashita, M. Shinohara, and A. Shinohara Rad6-Bre1-mediated histone H2B ubiquitylation modulates the formation of double-strand breaks during meiosis PNAS, August 3, 2004; 101(31): 11380 - 11385. [Abstract] [Full Text] [PDF]

				C. Shimoda Forespore membrane assembly in yeast: coordinating SPBs and membrane trafficking J. Cell Sci., January 22, 2004; 117(3): 389 - 396. [Abstract] [Full Text] [PDF]

				C. Wiederkehr, R. Basavaraj, C. Sarrauste de Menthiere, L. Hermida, R. Koch, U. Schlecht, A. Amon, S. Brachat, M. Breitenbach, P. Briza, et al. GermOnline, a cross-species community knowledgebase on germ cell differentiation Nucleic Acids Res., January 1, 2004; 32(90001): D560 - 567. [Abstract] [Full Text] [PDF]

				S. M. Honigberg and K. Purnapatre Signal pathway integration in the switch from the mitotic cell cycle to meiosis in yeast J. Cell Sci., June 1, 2003; 116(11): 2137 - 2147. [Abstract] [Full Text] [PDF]