Genetics, Vol. 163, 453-456, January 2003, Copyright © 2003

Mosaicism of Solid Gold Supports the Causality of a Noncoding A-to-G Transition in the Determinism of the Callipyge Phenotype

Maria Smita, Karin Segersb, Laura Garcia Carrascosab, Tracy Shaya, Francesca Baraldib, Gabor Gyapayc, Gary Snowderd, Michel Georgesb, Noelle Cocketta, and Carole Charlierb
a Department of Animal, Dairy and Veterinary Sciences, College of Agriculture, Utah State University, Logan, Utah 84322-4700,
b Department of Genetics, Faculty of Veterinary Medicine, University of Liège (B43), 4000-Liège, Belgium,
c Genoscope, Centre National de Séquençage, CP 5706, 91057 EVRY Cedex, France
d United States Department of Agriculture, Agricultural Research Station, U.S. Meat Animal Research Center, Clay Center, Nebraska 68933

Corresponding author: Michel Georges, Faculty of Veterinary Medicine, University of Liège (B43), 20 Bd. de Colonster, 4000-Liège, Belgium., michel.georges{at}ulg.ac.be (E-mail)

Communicating editor: C. HALEY

To identify the callipyge mutation, we have resequenced 184 kb spanning the DLK1-, GTL2-, PEG11-, and MEG8-imprinted domain and have identified an A-to-G transition in a highly conserved dodecamer motif between DLK1 and GTL2. This was the only difference found between the callipyge (CLPG) allele and a phylogenetically closely related wild-type allele. We report that this SNP is in perfect association with the callipyge genotype. The demonstration that Solid Gold—the alleged founder ram of the callipyge flock—is mosaic for this SNP virtually proves the causality of this SNP in the determinism of the callipyge phenotype.




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