Identification of a Functional Domain Within the Essential Core of Histone H3 That Is Required for Telomeric and HM Silencing in Saccharomyces cerevisiae

Identification of a Functional Domain Within the Essential Core of Histone H3 That Is Required for Telomeric and HM Silencing in Saccharomyces cerevisiae

Jeffrey S. Thompson^a, Marilyn L. Snow^a, Summer Giles^a, Leslie E. McPherson^a, and Michael Grunstein^b
^a Department of Biology, Georgian Court College, Lakewood, New Jersey 08701
^b Department of Biological Chemistry, UCLA School of Medicine and the Molecular Biology Institute, University of California, Los Angeles, California 90095

Corresponding author: Jeffrey S. Thompson, Georgian Court College, 900 Lakewood Ave., Lakewood, NJ 08701., thompsonj{at}georgian.edu (E-mail)

Communicating editor: L. PILLUS

Fourteen novel single-amino-acid substitution mutations in histoneH3 that disrupt telomeric silencing in Saccharomyces cerevisiaewere identified, 10 of which are clustered within the ${alpha}$ 1 helixand L1 loop of the essential histone fold. Several of thesemutations cause derepression of silent mating locus HML, andan additional subset cause partial loss of basal repressionat the GAL1 promoter. Our results identify a new domain withinthe essential core of histone H3 that is required for heterochromatin-mediatedsilencing.

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