Genetics, Vol. 163, 195-201, January 2003, Copyright © 2003

A P-Element Insertion Screen Identified Mutations in 455 Novel Essential Genes in Drosophila

Su-Wan Oha, Tracy Kingsleya, Hyun-hee Shina, Zhiyu Zhenga, Hua-Wei Chena, Xiu Chena, Hong Wanga, Peizheng Ruana, Michelle Moodya, and Steven X. Houa
a The Laboratory of Immunobiology, National Institutes of Health, National Cancer Institute, Frederick, Maryland 21702-1201

Corresponding author: Steven X. Hou, National Institutes of Health, National Cancer Institute, Frederick, MD 21702-1201., shou{at}mail.ncifcrf.gov (E-mail)

Communicating editor: T. SCHÜPBACH

With the completion of the nucleotide sequences of several complex eukaryotic genomes, tens of thousands of genes have been predicted. However, this information has to be correlated with the functions of those genes to enhance our understanding of biology and to improve human health care. The Drosophila transposon P-element-induced mutations are very useful for directly connecting gene products to their biological function. We designed an efficient transposon P-element-mediated gene disruption procedure and performed genetic screening for single P-element insertion mutations, enabling us to recover 2500 lethal mutations. Among these, 2355 are second chromosome mutations. Sequences flanking >2300 insertions that identify 850 different genes or ESTs (783 genes on the second chromosome and 67 genes on the third chromosome) have been determined. Among these, 455 correspond to genes for which no lethal mutation has yet been reported. The Drosophila genome is thought to contain ~3600 vital genes; 1400 are localized on the second chromosome. Our mutation collection represents ~56% of the second chromosome vital genes and ~24% of the total vital Drosophila genes.




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