help button home button Genetics J Biol Chem
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Formosa, T.
Right arrow Articles by Stillman, D. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Formosa, T.
Right arrow Articles by Stillman, D. J.
Genetics, Vol. 162, 1557-1571, December 2002, Copyright © 2002

Defects in SPT16 or POB3 (yFACT) in Saccharomyces cerevisiae Cause Dependence on the Hir/Hpc Pathway: Polymerase Passage May Degrade Chromatin Structure

Tim Formosaa, Susan Ruonea, Melissa D. Adamsc, Aileen E. Olsenb, Peter Erikssonb, Yaxin Yub, Alison R. Rhoadesa, Paul D. Kaufmanc, and David J. Stillmanb
a Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah 84132
b Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah 84132
c Lawrence Berkeley National Laboratory and Department of Molecular and Cell Biology, University of California, Berkeley, California 94720

Corresponding author: Tim Formosa, Biochemistry, 20 N 1900 E, Rm. 211, Salt Lake City, UT 84132-3201., Tim{at}biochem.utah.edu (E-mail)

Communicating editor: F. WINSTON

Spt16/Cdc68, Pob3, and Nhp6 collaborate in vitro and in vivo as the yeast factor SPN, which is homologous to human FACT. SPN/FACT complexes mediate passage of polymerases through nucleosomes and are important for both transcription and replication. An spt16 mutation was found to be intolerable when combined with a mutation in any member of the set of functionally related genes HIR1, HIR2/SPT1, HIR3/HPC1, or HPC2. Mutations in POB3, but not in NHP6A/B, also display strong synthetic defects with hir/hpc mutations. A screen for other mutations that cause dependence on HIR/HPC genes revealed genes encoding members of the Paf1 complex, which also promotes transcriptional elongation. The Hir/Hpc proteins affect the expression of histone genes and also promote normal deposition of nucleosomes; either role could explain an interaction with elongation factors. We show that both spt16 and pob3 mutants respond to changes in histone gene numbers, but in opposite ways, suggesting that Spt16 and Pob3 each interact with histones but perhaps with different subsets of these proteins. Supporting this, spt16 and pob3 mutants also display different sensitivities to mutations in the N-terminal tails of histones H3 and H4 and to mutations in enzymes that modulate acetylation of these tails. Our results support a model in which SPN/FACT has two functions: it disrupts nucleosomes to allow polymerases to access DNA, and it reassembles the nucleosomes afterward. Mutations that impair the reassembly activity cause chromatin to accumulate in an abnormally disrupted state, imposing a requirement for a nucleosome reassembly function that we propose is provided by Hir/Hpc proteins.




This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
D. Biswas, S. Takahata, and D. J. Stillman
Different Genetic Functions for the Rpd3(L) and Rpd3(S) Complexes Suggest Competition between NuA4 and Rpd3(S)
Mol. Cell. Biol., July 15, 2008; 28(14): 4445 - 4458.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T. Stuwe, M. Hothorn, E. Lejeune, V. Rybin, M. Bortfeld, K. Scheffzek, and A. G. Ladurner
The FACT Spt16 "peptidase" domain is a histone H3-H4 binding module
PNAS, July 1, 2008; 105(26): 8884 - 8889.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
A. Gradolatto, R. S. Rogers, H. Lavender, S. D. Taverna, C. D. Allis, J. D. Aitchison, and A. J. Tackett
Saccharomyces cerevisiae Yta7 Regulates Histone Gene Expression
Genetics, May 1, 2008; 179(1): 291 - 304.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. P. VanDemark, H. Xin, L. McCullough, R. Rawlins, S. Bentley, A. Heroux, D. J. Stillman, C. P. Hill, and T. Formosa
Structural and Functional Analysis of the Spt16p N-terminal Domain Reveals Overlapping Roles of yFACT Subunits
J. Biol. Chem., February 22, 2008; 283(8): 5058 - 5068.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
D. Biswas, S. Takahata, H. Xin, R. Dutta-Biswas, Y. Yu, T. Formosa, and D. J. Stillman
A Role for Chd1 and Set2 in Negatively Regulating DNA Replication in Saccharomyces cerevisiae
Genetics, February 1, 2008; 178(2): 649 - 659.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
D. Biswas, R. Dutta-Biswas, and D. J. Stillman
Chd1 and yFACT Act in Opposition in Regulating Transcription
Mol. Cell. Biol., September 15, 2007; 27(18): 6279 - 6287.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
A. A. Duina, A. Rufiange, J. Bracey, J. Hall, A. Nourani, and F. Winston
Evidence that the Localization of the Elongation Factor Spt16 Across Transcribed Genes Is Dependent Upon Histone H3 Integrity in Saccharomyces cerevisiae
Genetics, September 1, 2007; 177(1): 101 - 112.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
V. M. Sharma, R. S. Tomar, A. E. Dempsey, and J. C. Reese
Histone Deacetylases RPD3 and HOS2 Regulate the Transcriptional Activation of DNA Damage-Inducible Genes
Mol. Cell. Biol., April 15, 2007; 27(8): 3199 - 3210.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
S. Kim, M. J. Wolyniak, J. F. Staab, and P. Sundstrom
A 368-Base-Pair cis-Acting HWP1 Promoter Region, HCR, of Candida albicans Confers Hypha-Specific Gene Regulation and Binds Architectural Transcription Factors Nhp6 and Gcf1p
Eukaryot. Cell, April 1, 2007; 6(4): 693 - 709.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Li, S. X. Zeng, I. Landais, and H. Lu
Human SSRP1 Has Spt16-dependent and -independent Roles in Gene Transcription
J. Biol. Chem., March 9, 2007; 282(10): 6936 - 6945.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S. Jimeno-Gonzalez, F. Gomez-Herreros, P. M. Alepuz, and S. Chavez
A Gene-Specific Requirement for FACT during Transcription Is Related to the Chromatin Organization of the Transcribed Region
Mol. Cell. Biol., December 1, 2006; 26(23): 8710 - 8721.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Reinberg and R. J. Sims III
de FACTo Nucleosome Dynamics
J. Biol. Chem., August 18, 2006; 281(33): 23297 - 23301.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Greenall, E. S. Williams, K. A. Martin, J. M. Palmer, J. Gray, C. Liu, and S. K. Whitehall
Hip3 Interacts with the HIRA Proteins Hip1 and Slm9 and Is Required for Transcriptional Silencing and Accurate Chromosome Segregation
J. Biol. Chem., March 31, 2006; 281(13): 8732 - 8739.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
A. Nourani, F. Robert, and F. Winston
Evidence that Spt2/Sin1, an HMG-Like Factor, Plays Roles in Transcription Elongation, Chromatin Structure, and Genome Stability in Saccharomyces cerevisiae
Mol. Cell. Biol., February 15, 2006; 26(4): 1496 - 1509.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
K. Adelman, W. Wei, M. B. Ardehali, J. Werner, B. Zhu, D. Reinberg, and J. T. Lis
Drosophila Paf1 Modulates Chromatin Structure at Actively Transcribed Genes
Mol. Cell. Biol., January 1, 2006; 26(1): 250 - 260.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
D. Prather, N. J. Krogan, A. Emili, J. F. Greenblatt, and F. Winston
Identification and Characterization of Elf1, a Conserved Transcription Elongation Factor in Saccharomyces cerevisiae
Mol. Cell. Biol., November 15, 2005; 25(22): 10122 - 10135.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
E. B. Gomez, J. M. Espinosa, and S. L. Forsburg
Schizosaccharomyces pombe mst2+ Encodes a MYST Family Histone Acetyltransferase That Negatively Regulates Telomere Silencing
Mol. Cell. Biol., October 15, 2005; 25(20): 8887 - 8903.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
D. Biswas, Y. Yu, M. Prall, T. Formosa, and D. J. Stillman
The Yeast FACT Complex Has a Role in Transcriptional Initiation
Mol. Cell. Biol., July 15, 2005; 25(14): 5812 - 5822.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. E. Kong, M. S. Kobor, N. J. Krogan, B. P. Somesh, T. M. M. Sogaard, J. F. Greenblatt, and J. Q. Svejstrup
Interaction of Fcp1 Phosphatase with Elongating RNA Polymerase II Holoenzyme, Enzymatic Mechanism of Action, and Genetic Interaction with Elongator
J. Biol. Chem., February 11, 2005; 280(6): 4299 - 4306.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. D. Kaplan, M. J. Holland, and F. Winston
Interaction between Transcription Elongation Factors and mRNA 3'-End Formation at the Saccharomyces cerevisiae GAL10-GAL7 Locus
J. Biol. Chem., January 14, 2005; 280(2): 913 - 922.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
Y. Zhou and T. S.-F. Wang
A Coordinated Temporal Interplay of Nucleosome Reorganization Factor, Sister Chromatin Cohesion Factor, and DNA Polymerase {alpha} Facilitates DNA Replication
Mol. Cell. Biol., November 1, 2004; 24(21): 9568 - 9579.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. F. O'Donnell, N. K. Brewster, J. Kurniawan, L. V. Minard, G. C. Johnston, and R. A. Singer
Domain organization of the yeast histone chaperone FACT: the conserved N-terminal domain of FACT subunit Spt16 mediates recovery from replication stress
Nucleic Acids Res., November 1, 2004; 32(19): 5894 - 5906.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
R. J. Sims III, R. Belotserkovskaya, and D. Reinberg
Elongation by RNA polymerase II: the short and long of it
Genes & Dev., October 15, 2004; 18(20): 2437 - 2468.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
K. Matsumoto and S. Hirose
Visualization of unconstrained negative supercoils of DNA on polytene chromosomes of Drosophila
J. Cell Sci., August 1, 2004; 117(17): 3797 - 3805.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. G. Petrakis, B. O. Wittschieben, and J. Q. Svejstrup
Molecular Architecture, Structure-Function Relationship, and Importance of the Elp3 Subunit for the RNA Binding of Holo-Elongator
J. Biol. Chem., July 30, 2004; 279(31): 32087 - 32092.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
A. R. Rhoades, S. Ruone, and T. Formosa
Structural Features of Nucleosomes Reorganized by Yeast FACT and Its HMG Box Component, Nhp6
Mol. Cell. Biol., May 1, 2004; 24(9): 3907 - 3917.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. C.-M. Tan and S.-C. Lee
Nek9, a Novel FACT-associated Protein, Modulates Interphase Progression
J. Biol. Chem., March 5, 2004; 279(10): 9321 - 9330.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
K. M. Robinson and M. C. Schultz
Replication-Independent Assembly of Nucleosome Arrays in a Novel Yeast Chromatin Reconstitution System Involves Antisilencing Factor Asf1p and Chromodomain Protein Chd1p
Mol. Cell. Biol., November 15, 2003; 23(22): 7937 - 7946.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
P. B. Mason and K. Struhl
The FACT Complex Travels with Elongating RNA Polymerase II and Is Important for the Fidelity of Transcriptional Initiation In Vivo
Mol. Cell. Biol., November 15, 2003; 23(22): 8323 - 8333.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Ruone, A. R. Rhoades, and T. Formosa
Multiple Nhp6 Molecules Are Required to Recruit Spt16-Pob3 to Form yFACT Complexes and to Reorganize Nucleosomes
J. Biol. Chem., November 14, 2003; 278(46): 45288 - 45295.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
R. Jansen, H. Yu, D. Greenbaum, Y. Kluger, N. J. Krogan, S. Chung, A. Emili, M. Snyder, J. F. Greenblatt, and M. Gerstein
A Bayesian Networks Approach for Predicting Protein-Protein Interactions from Genomic Data
Science, October 17, 2003; 302(5644): 449 - 453.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
R. Belotserkovskaya, S. Oh, V. A. Bondarenko, G. Orphanides, V. M. Studitsky, and D. Reinberg
FACT Facilitates Transcription-Dependent Nucleosome Alteration
Science, August 22, 2003; 301(5636): 1090 - 1093.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
A. Saunders, J. Werner, E. D. Andrulis, T. Nakayama, S. Hirose, D. Reinberg, and J. T. Lis
Tracking FACT and the RNA Polymerase II Elongation Complex Through Chromatin in Vivo
Science, August 22, 2003; 301(5636): 1094 - 1096.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
C. D. Kaplan, L. Laprade, and F. Winston
Transcription Elongation Factors Repress Transcription Initiation from Cryptic Sites
Science, August 22, 2003; 301(5636): 1096 - 1099.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2002 by the Genetics Society of America.