Cold Sweetening in Diploid Potato: Mapping Quantitative Trait Loci and Candidate Genes

Cold Sweetening in Diploid Potato: Mapping Quantitative Trait Loci and Candidate Genes

Cristina M. Menéndez^a, Enrique Ritter^b, Ralf Schäfer-Pregl^a, Birgit Walkemeier^a, Alexandra Kalde^a, Francesco Salamini^a, and Christiane Gebhardt^a
^a Max-Planck-Institut für Züchtungsforschung, 50829 Köln, Germany
^b NEIKER, Granja Modelo de Arkaute, 01080 Vitoria, Spain

Corresponding author: Christiane Gebhardt, Carl-von-Linné-Weg 10, 50829 Köln, Germany., gebhardt{at}mpiz-koeln.mpg.de (E-mail)

Communicating editor: A. H. D. BROWN

A candidate gene approach has been used as a first step to identifythe molecular basis of quantitative trait variation in potato.Sugar content of tubers upon cold storage was the model traitchosen because the metabolic pathways involved in starch andsugar metabolism are well known and many of the genes have beencloned. Tubers of two F₁ populations of diploid potato grownin six environments were evaluated for sugar content after coldstorage. The populations were genotyped with RFLP, AFLP, andcandidate gene markers. QTL analysis revealed that QTL for glucose,fructose, and sucrose content were located on all potato chromosomes.Most QTL for glucose content mapped to the same positions asQTL for fructose content. QTL explaining >10% of the variabilityfor reducing sugars were located on linkage groups I, III, VII,VIII, IX, and XI. QTL consistent across populations and/or environmentswere identified. QTL were linked to genes encoding invertase,sucrose synthase 3, sucrose phosphate synthase, ADP-glucosepyrophosphorylase, sucrose transporter 1, and a putative sucrosesensor. The results suggest that allelic variants of enzymesoperating in carbohydrate metabolic pathways contribute to thegenetic variation in cold sweetening.

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