Identification of 1088 New Transposon Insertions of Caenorhabditis elegans: A Pilot Study Toward Large-Scale Screens

Identification of 1088 New Transposon Insertions of Caenorhabditis elegans: A Pilot Study Toward Large-Scale Screens

Edwige Martin^a, Hélène Laloux^a, Gaëlle Couette^a, Thierry Alvarez^a, Catherine Bessou^a, Oliver Hauser^a, Satis Sookhareea^b, Michel Labouesse^b, and Laurent Ségalat^a
^a CGMC, CNRS-UMR 5534, Université Lyon1, 69100 Villeurbanne, France
^b IGBMC, BP. 163, 67404 Illkirch, France

Corresponding author: Laurent Ségalat, Université Lyon1, 43 bld du 11 Novembre, 69622 Villeurbanne Cedex, France., segalat{at}maccgmc.univ-lyon1.fr (E-mail)

Communicating editor: P. ANDERSON

We explored the feasibility of a strategy based on transposonsto generate identified mutants of most Caenorhabditis elegansgenes. A total of 1088 random new insertions of C. elegans transposonsTc1, Tc3, and Tc5 were identified by anchored PCR, some of whichresult in a mutant phenotype.

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