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Genetics, Vol. 162, 285-296, September 2002, Copyright © 2002

Genetic Interaction Between Integrins and moleskin, a Gene Encoding a Drosophila Homolog of Importin-7

Scott E. Bakera, James A. Lorenzenc, Steven W. Millera, Thomas A. Buncha, Alison L. Jannuzia, Mark H. Ginsbergd, Lizabeth A. Perkinsc, and Danny L. Browera,b
a Department of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona 85721,
b Department of Biochemistry, University of Arizona, Tucson, Arizona 85721,
c Pediatric Surgical Research Laboratories, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts 02129
d Department of Vascular Biology, The Scripps Research Institute, La Jolla, California 92037

Corresponding author: Danny L. Brower, Life Sciences South Bldg., 1007 E. Lowell St., University of Arizona, Tucson, AZ 85721., dbrower{at}u.arizona.edu (E-mail)

Communicating editor: T. CLINE

The Drosophila PS1 and PS2 integrins are required to maintain the connection between the dorsal and ventral wing epithelia. If {alpha}PS subunits are inappropriately expressed during early pupariation, the epithelia separate, causing a wing blister. Two lines of evidence indicate that this apparent loss-of-function phenotype is not a dominant negative effect, but is due to inappropriate expression of functional integrins: wing blisters are not generated efficiently by misexpression of loss-of-function {alpha}PS2 subunits with mutations that inhibit ligand binding, and gain-of-function, hyperactivated mutant {alpha}PS2 proteins cause blistering at expression levels well below those required by wild-type proteins. A genetic screen for dominant suppressors of wing blisters generated null alleles of a gene named moleskin, which encodes the protein DIM-7. DIM-7, a Drosophila homolog of vertebrate importin-7, has recently been shown to bind the SHP-2 tyrosine phosphatase homolog Corkscrew and to be important in the nuclear translocation of activated D-ERK. Consistent with this latter finding, homozygous mutant clones of moleskin fail to grow in the wing. Genetic tests suggest that the moleskin suppression of wing blisters is not directly related to inhibition of D-ERK nuclear import. These data are discussed with respect to the possible regulation of integrin function by cytoplasmic ERK.




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