Using the P{wHy} Hybrid Transposable Element to Disrupt Genes in Region 54D-55B in Drosophila melanogaster

Using the P{wHy} Hybrid Transposable Element to Disrupt Genes in Region 54D-55B in Drosophila melanogaster

Stephanie E. Mohr^a and William M. Gelbart^a
^a Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138

Corresponding author: William M. Gelbart, Cambridge, MA 02138., gelbart{at}morgan.harvard.edu (E-mail)

Communicating editor: T. SCHÜPBACH

Understanding the function of each gene in the genome of a modelorganism such as Drosophila melanogaster is an important goal.The development of improved methods for uncovering the mutantphenotypes of specific genes can accelerate achievement of thisgoal. The P{wHy} hybrid transposable element can be used togenerate nested sets of precisely mapped deletions in a givenregion of the Drosophila genome. Here we use the P{wHy} methodto generate overlapping, molecularly defined deletions froma set of three P{wHy} insertions in the 54E-F region of chromosome2. Deletions that span a total of 0.5 Mb were identified andmolecularly mapped precisely. Using overlapping deletions, themutant phenotypes of nine previously uncharacterized genes ina 101-kb region were determined, including identification ofnew loci required for viability and female fertility. In addition,the deletions were used to molecularly map previously isolatedlethal mutations. Thus, the P{wHy} method provides an efficientmethod for systematically determining the phenotypes of genesin a given region of the fly genome.

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