Genetics, Vol. 162, 129-134, September 2002, Copyright © 2002

A Bacterial Artificial Chromosome-Based Genetic Linkage Map of the Nematode Pristionchus pacificus

Jagan Srinivasana, Waltraud Sinza, Christa Lanzb, Alexandra Branda, Ramkumar Nandakumarb, Günter Raddatzb, Hanh Wittea, Heike Kellerb, Isabel Kippinga, André Pires-daSilvaa, Taco Jessec, Jun Millarec, Michiel de Bothc, Stephan C. Schusterb, and Ralf J. Sommera
a Abteilung für Evolutionsbiologie, Max-Planck Institut für Entwicklungsbiologie, 72076 Tübingen, Germany,
b Genomzentrum, Max-Planck Institut für Entwicklungsbiologie, 72076 Tübingen, Germany
c Keygene N.V., 6708 AE Wageningen, The Netherlands

Corresponding author: Ralf J. Sommer, Max-Planck Institute for Developmental Biology, Spemannstrasse 37, D-72076 Tübingen, Germany., ralf.sommer{at}tuebingen.mpg.de (E-mail)

Communicating editor: D. KINGSLEY

To understand the evolution of developmental processes, nonmodel organisms in the nematodes, insects, and vertebrates are compared with established model systems. Often, these comparisons suffer from the inability to apply sophisticated technologies to these nonmodel species. In the nematode Pristionchus pacificus, cellular and genetic analyses are used to compare vulva development to that of Caenorhabditis elegans. However, substantial changes in gene function between P. pacificus and C. elegans limit the use of candidate gene approaches in studying P. pacificus mutations. To facilitate map-based cloning of mutations in P. pacificus, we constructed a BAC-based genetic linkage map. A BAC library of 13,440 clones was generated and completely end sequenced. By comparing BAC end and EST sequences between the "wild-type" strain P. pacificus var. California and the polymorphic strain P. pacificus var. Washington, 133 single-stranded conformational polymorphisms were identified. These markers were tested on a meiotic mapping panel of 46 randomly picked F2 animals after a cross of the two strains, providing the first genetic linkage map of P. pacificus. A mapping strategy using two selected markers per chromosome was devised and the efficiency of this approach was illustrated by the mapping of the Ppa-unc-1/Twitchin gene.




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