Genetics, Vol. 161, 983-994, July 2002, Copyright © 2002

An {alpha}-Tubulin Mutant Demonstrates Distinguishable Functions Among the Spindle Assembly Checkpoint Genes in Saccharomyces cerevisiae

Katharine C. Abruzzib, Margaret Magendantza, and Frank Solomona
a Department of Biology and Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
b Department of Biology, Brandeis University, Waltham, Massachusetts 02454

Corresponding author: Frank Solomon, Rm. 220, Massachusetts Institute of Technology, Cambridge, MA 02139., solomon{at}mit.edu (E-mail)

Communicating editor: M. D. ROSE

Cells expressing a mutant allele of {alpha}-tubulin, tub1-729, are cold sensitive and arrest as large-budded cells with microtubule defects. The cold sensitivity of tub1-729 is suppressed by extra copies of a subset of the mitotic checkpoint genes BUB1, BUB3, and MPS1, but not MAD1, MAD2, and MAD3. This suppression by checkpoint genes does not depend upon their role in the MAD2-dependent spindle assembly checkpoint. In addition, BUB1 requires an intact kinase domain as well as Bub3p to suppress tub1-729. The data suggest that tub1-729 cells are defective in microtubule-kinetochore attachments and that the products of specific checkpoint genes can act either directly or indirectly to affect these attachments.




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