Genetics, Vol. 161, 1247-1255, July 2002, Copyright © 2002

A Screen for Genes That Function in Abscisic Acid Signaling in Arabidopsis thaliana

Eiji Nambaraa,b, Masaharu Suzukic, Suzanne Abramsd, Donald R. McCartyc, Yuji Kamiyab, and Peter McCourta
a Department of Botany, University of Toronto, Toronto, Ontario M5S 3B2, Canada,
b Plant Science Center, The Institute of Physical and Chemical Research (RIKEN), Wako, Japan 351-0198,
c Horticultural Sciences Department, University of Florida, Gainesville, Florida 32605
d Plant Biotechnology Institute, National Research Council of Canada, Saskatoon, Saskatchewan S7N 0W9, Canada

Corresponding author: Peter McCourt, University of Toronto, 25 Willcocks St., Toronto, ON M5S 3B2, Canada., mccourt{at}botany.utoronto.ca (E-mail)

Communicating editor: C. S. GASSER

The plant hormone abscisic acid (ABA) controls many aspects of plant growth and development under a diverse range of environmental conditions. To identify genes functioning in ABA signaling, we have carried out a screen for mutants that takes advantage of the ability of wild-type Arabidopsis seeds to respond to (-)-(R)-ABA, an enantiomer of the natural (+)-(S)-ABA. The premise of the screen was to identify mutations that preferentially alter their germination response in the presence of one stereoisomer vs. the other. Twenty-six mutants were identified and genetic analysis on 23 lines defines two new loci, designated CHOTTO1 and CHOTTO2, and a collection of new mutant alleles of the ABA-insensitive genes, ABI3, ABI4, and ABI5. The abi5 alleles are less sensitive to (+)-ABA than to (-)-ABA. In contrast, the abi3 alleles exhibit a variety of differences in response to the ABA isomers. Genetic and molecular analysis of these alleles suggests that the ABI3 transcription factor may perceive multiple ABA signals.




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