Genetics, Vol. 161, 1225-1234, July 2002, Copyright © 2002

Integration of the Cytogenetic and Genetic Linkage Maps of Brassica oleracea

Elaine C. Howella, Guy C. Barkerb, Gareth H. Jonesa, Michael J. Kearseya, Graham J. Kingb, Erik P. Kopb, Carol D. Ryderb, Graham R. Teakleb, Joana G. Vicenteb, and Susan J. Armstronga
a School of Biosciences, The University of Birmingham, Birmingham B15 2TT, United Kingdom
b Horticulture Research International, Wellesbourne, Warwickshire CV35 9EF, United Kingdom

Corresponding author: Susan J. Armstrong, The University of Birmingham, Edgbaston, Birmingham B15 2TT, UK., s.j.armstrong{at}bham.ac.uk (E-mail)

Communicating editor: C. HALEY

We have assigned all nine linkage groups of a Brassica oleracea genetic map to each of the nine chromosomes of the karyotype derived from mitotic metaphase spreads of the B. oleracea var. alboglabra line A12DHd using FISH. The majority of probes were BACs, with A12DHd DNA inserts, which give clear, reliable FISH signals. We have added nine markers to the existing integrated linkage map, distributed over six linkage groups. BACs were definitively assigned to linkage map positions through development of locus-specific PCR assays. Integration of the cytogenetic and genetic linkage maps was achieved with 22 probes representing 19 loci. Four chromosomes (2, 4, 7, and 9) are in the same orientation as their respective linkage groups (O4, O7, O8, and O6) whereas four chromosomes (1, 3, 5, and 8) and linkage groups (O3, O9, O2, and O1) are in the opposite orientation. The remaining chromosome (6) is probably in the opposite orientation. The cytogenetic map is an important resource for locating probes with unknown genetic map positions and is also being used to analyze the relationships between genetic and cytogenetic maps.




This article has been cited by other articles:


Home page
 GeneticsHome page
F. I. E. Amarillo and H. W. Bass
A Transgenomic Cytogenetic Sorghum (Sorghum propinquum) Bacterial Artificial Chromosome Fluorescence in Situ Hybridization Map of Maize (Zea mays L.) Pachytene Chromosome 9, Evidence for Regions of Genome Hyperexpansion
Genetics, November 1, 2007; 177(3): 1509 - 1526.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
C.-J. R. Wang, L. Harper, and W. Z. Cande
High-Resolution Single-Copy Gene Fluorescence in Situ Hybridization and Its Use in the Construction of a Cytogenetic Map of Maize Chromosome 9
PLANT CELL, March 1, 2006; 18(3): 529 - 544.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
R. HASTEROK, E. WOLNY, M. HOSIAWA, M. KOWALCZYK, S. KULAK-KSIAZCZYK, T. KSIAZCZYK, W. K. HENEEN, and J. MALUSZYNSKA
Comparative Analysis of rDNA Distribution in Chromosomes of Various Species of Brassicaceae
Ann. Bot., February 1, 2006; 97(2): 205 - 216.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
I. A. P. Parkin, S. M. Gulden, A. G. Sharpe, L. Lukens, M. Trick, T. C. Osborn, and D. J. Lydiate
Segmental Structure of the Brassica napus Genome Based on Comparative Analysis With Arabidopsis thaliana
Genetics, October 1, 2005; 171(2): 765 - 781.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
J. A. Udall, P. A. Quijada, and T. C. Osborn
Detection of Chromosomal Rearrangements Derived From Homeologous Recombination in Four Mapping Populations of Brassica napus L.
Genetics, February 1, 2005; 169(2): 967 - 979.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
J.-S. Kim, P. E. Klein, R. R. Klein, H. J. Price, J. E. Mullet, and D. M. Stelly
Chromosome Identification and Nomenclature of Sorghum bicolor
Genetics, February 1, 2005; 169(2): 1169 - 1173.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
T. C. Osborn, D. V. Butrulle, A. G. Sharpe, K. J. Pickering, I. A. P. Parkin, J. S. Parker, and D. J. Lydiate
Detection and Effects of a Homeologous Reciprocal Transposition in Brassica napus
Genetics, November 1, 2003; 165(3): 1569 - 1577.
[Abstract] [Full Text] [PDF]