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Genetics, Vol. 161, 585-594, June 2002, Copyright © 2002

Gene Overexpression as a Tool for Identifying New trans-Acting Factors Involved in Translation Termination in Saccharomyces cerevisiae

Olivier Namya, Isabelle Hatina, Guillaume Stahla, Hongmei Liua, Stephanie Barnaya, Laure Bidoua, and Jean-Pierre Rousseta
a Laboratoire de Génétique Moléculaire de la Traduction, Institut de Génétique et Microbiologie, CNRS UMR8621, Université Paris-Sud, 91405 Orsay Cedex, France

Corresponding author: Jean-Pierre Rousset, Institut de Génétique et Microbiologie, bâtiment 400, Université Paris-Sud, 91405 Orsay Cedex, France., rousset{at}igmors.u-psud.fr (E-mail)

Communicating editor: A. NICOLAS

In eukaryotes, translation termination is dependent on the availability of both release factors, eRF1 and eRF3; however, the precise mechanisms involved remain poorly understood. In particular, the fact that the phenotype of release factor mutants is pleiotropic could imply that other factors and interactions are involved in translation termination. To identify unknown elements involved in this process, we performed a genetic screen using a reporter strain in which a leaky stop codon is inserted in the lacZ reporter gene, attempting to isolate factors modifying termination efficiency when overexpressed. Twelve suppressors and 11 antisuppressors, increasing or decreasing termination readthrough, respectively, were identified and analyzed for three secondary phenotypes often associated with translation mutations: thermosensitivity, G418 sensitivity, and sensitivity to osmotic pressure. Interestingly, among these candidates, we identified two genes, SSO1 and STU2, involved in protein transport and spindle pole body formation, respectively, suggesting puzzling connections with the translation termination process.




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Copyright © 2002 by the Genetics Society of America.