| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Corresponding author: Alain Nicolas, Section de recherche, CNRS UMR144 26, rue d'Ulm, 74248 Paris Cedex 05, France., alain.nicolas{at}curie.fr (E-mail)
Communicating editor: L. S. SYMINGTON
3' resection to produce 3' single-stranded DNA ends that serve to channel DSBs into the RAD52 recombinational repair pathway. In vitro studies strongly suggest that several proteins of this pathway—Rad51, Rad52, Rad54, Rad55, Rad57, and replication protein A (RPA)—play a role in the strand exchange reaction. Here, we report a study of the meiotic phenotypes conferred by two missense mutations affecting the largest subunit of RPA, which are localized in the protein interaction domain (rfa1-t11) and in the DNA-binding domain (rfa1-t48). We find that both mutant diploids exhibit reduced sporulation efficiency, very poor spore viability, and a 10- to 100-fold decrease in meiotic recombination. Physical analyses indicate that both mutants form normal levels of meiosis-specific DSBs and that the broken ends are processed into 3'-OH single-stranded tails, indicating that the RPA complex present in these rfa1 mutants is functional in the initial steps of meiotic recombination. However, the 5' ends of the broken fragments undergo extensive resection, similar to what is observed in rad51, rad52, rad55, and rad57 mutants, indicating that these RPA mutants are defective in the repair of the Spo11-dependent DSBs that initiate homologous recombination during meiosis.
This article has been cited by other articles:
 |
|
 |
|
  S. J. Haring, A. C. Mason, S. K. Binz, and M. S. Wold Cellular Functions of Human RPA1: MULTIPLE ROLES OF DOMAINS IN REPLICATION, REPAIR, AND CHECKPOINTS J. Biol. Chem., July 4, 2008; 283(27): 19095 - 19111. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Mozlin, C. W. Fung, and L. S. Symington Role of the Saccharomyces cerevisiae Rad51 Paralogs in Sister Chromatid Recombination Genetics, January 1, 2008; 178(1): 113 - 126. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. L. Ball, M. R. Ehrhardt, D. A. Mordes, G. G. Glick, W. J. Chazin, and D. Cortez Function of a Conserved Checkpoint Recruitment Domain in ATRIP Proteins Mol. Cell. Biol., May 1, 2007; 27(9): 3367 - 3377. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Ploquin, G. V. Petukhova, D. Morneau, U. Dery, A. Bransi, A. Stasiak, R. D. Camerini-Otero, and J.-Y. Masson Stimulation of fission yeast and mouse Hop2-Mnd1 of the Dmc1 and Rad51 recombinases Nucleic Acids Res., April 10, 2007; (2007) gkm174v1. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Majka, S. K. Binz, M. S. Wold, and P. M. J. Burgers Replication Protein A Directs Loading of the DNA Damage Checkpoint Clamp to 5'-DNA Junctions J. Biol. Chem., September 22, 2006; 281(38): 27855 - 27861. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Lopes, C. Ribeyre, and A. Nicolas Complex Minisatellite Rearrangements Generated in the Total or Partial Absence of Rad27/hFEN1 Activity Occur in a Single Generation and Are Rad51 and Rad52 Dependent. Mol. Cell. Biol., September 1, 2006; 26(17): 6675 - 6689. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-J. Park, J.-H. Lee, and B.-S. Choi Solution structure of the DNA-binding domain of RPA from Saccharomyces cerevisiae and its interaction with single-stranded DNA and SV40 T antigen Nucleic Acids Res., July 25, 2005; 33(13): 4172 - 4181. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Young, R. W. Hyppa, and G. R. Smith Conserved and Nonconserved Proteins for Meiotic DNA Breakage and Repair in Yeasts Genetics, June 1, 2004; 167(2): 593 - 605. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. K. Binz, Y. Lao, D. F. Lowry, and M. S. Wold The Phosphorylation Domain of the 32-kDa Subunit of Replication Protein A (RPA) Modulates RPA-DNA Interactions: EVIDENCE FOR AN INTERSUBUNIT INTERACTION J. Biol. Chem., September 12, 2003; 278(37): 35584 - 35591. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Kantake, T. Sugiyama, R. D. Kolodner, and S. C. Kowalczykowski The Recombination-deficient Mutant RPA (rfa1-t11) Is Displaced Slowly from Single-stranded DNA by Rad51 Protein J. Biol. Chem., June 20, 2003; 278(26): 23410 - 23417. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. S. Symington Role of RAD52 Epistasis Group Genes in Homologous Recombination and Double-Strand Break Repair Microbiol. Mol. Biol. Rev., December 1, 2002; 66(4): 630 - 670. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. L. Eggler, R. B. Inman, and M. M. Cox The Rad51-dependent Pairing of Long DNA Substrates Is Stabilized by Replication Protein A J. Biol. Chem., October 11, 2002; 277(42): 39280 - 39288. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
