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Alterations of the Portal Protein, gpB, of Bacteriophage 
Suppress Mutations in cosQ, the Site Required for Termination of DNA Packaging
Douglas J. Wieczoreka,
Lisa Didiona, and
Michael Feissa
a Genetics Ph.D. Program and Department of Microbiology, University of Iowa, Iowa City, Iowa 52242
Corresponding author: Douglas J. Wieczorek, University of Iowa, 3-315 BSB, 51 Newton Rd., Iowa City, IA 52242., wieczorekd{at}mail.medicine.uiowa.edu (E-mail)
Communicating editor: G. R. SMITH
is required for DNA packaging termination. Previous studies have shown that cosQ mutations can be suppressed in three ways: by a local suppressor within cosQ, an increase in the length of the chromosome, and missense mutations affecting the prohead's portal protein, gpB. In the present work, revertants of a set of lethal cosQ mutants were screened for suppressors. Seven new cosQ suppressors affected gene B, which encodes the portal protein of the prohead. All seven were allele-nonspecific suppressors of cosQ mutations. Experiments with several phages having two cosQ suppressors showed that the suppression effects were additive. Furthermore, these double suppressors had minimal effects on the growth of cosQ+ phages. These trans-acting suppressors affecting the portal protein are proposed to allow the mutant cosQ site to be more efficiently recognized, due to the slowing of the rate of translocation.
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  D. J. Wieczorek and M. Feiss Genetics of cosQ, the DNA-Packaging Termination Site of Phage {lambda}: Local Suppressors and Methylation Effects Genetics, September 1, 2003; 165(1): 11 - 21. [Abstract] [Full Text] [PDF]
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