Isolation and Characterization of Broad-Spectrum Disease-Resistant Arabidopsis Mutants

Corresponding author: Jeffery L. Dangl, 108 Coker Hall CB 3280, University of North Carolina, Chapel Hill, NC 27599-3280., dangl{at}email.unc.edu (E-mail)

Communicating editor: V. L. CHANDLER

To identify Arabidopsis mutants that constitutively express systemic acquired resistance (SAR), we constructed reporter lines expressing the firefly luciferase gene under the control of the SAR-inducible PR-1 promoter (PR-1/luc). After EMS mutagenesis of a well-characterized transgenic line, we screened 250,000 M₂ plants for constitutive expression of the reporter gene in vivo. From a mutant collection containing several hundred putative mutants, we concentrated on 16 mutants lacking spontaneous hypersensitive response (HR) cell death. We mapped 4 of these constitutive immunity (cim) mutants to chromosome arms. Constitutive expression of disease resistance was established by analyzing responses to virulent Peronospora parasitica and Pseudomonas syringae strains, by RNA blot analysis for endogenous marker genes, and by determination of salicylic acid levels in the mutants. The variety of the cim phenotypes allowed us to define distinct steps in both the canonical SAR signaling pathway and a separate pathway for resistance to Erysiphe cichoracearum, active in only a subset of the mutants.

This article has been cited by other articles:

				G. Fabro, J. A. Di Rienzo, C. A. Voigt, T. Savchenko, K. Dehesh, S. Somerville, and M. E. Alvarez Genome-Wide Expression Profiling Arabidopsis at the Stage of Golovinomyces cichoracearum Haustorium Formation Plant Physiology, March 1, 2008; 146(3): 1421 - 1439. [Abstract] [Full Text] [PDF]

				S. I. Zarate, L. A. Kempema, and L. L. Walling Silverleaf Whitefly Induces Salicylic Acid Defenses and Suppresses Effectual Jasmonic Acid Defenses Plant Physiology, February 1, 2007; 143(2): 866 - 875. [Abstract] [Full Text] [PDF]

				R. A. Mosher, W. E. Durrant, D. Wang, J. Song, and X. Dong A Comprehensive Structure-Function Analysis of Arabidopsis SNI1 Defines Essential Regions and Transcriptional Repressor Activity PLANT CELL, July 1, 2006; 18(7): 1750 - 1765. [Abstract] [Full Text] [PDF]

				W. Sun, F. M. Dunning, C. Pfund, R. Weingarten, and A. F. Bent Within-Species Flagellin Polymorphism in Xanthomonas campestris pv campestris and Its Impact on Elicitation of Arabidopsis FLAGELLIN SENSING2-Dependent Defenses PLANT CELL, March 1, 2006; 18(3): 764 - 779. [Abstract] [Full Text] [PDF]

				M. Stein, J. Dittgen, C. Sanchez-Rodriguez, B.-H. Hou, A. Molina, P. Schulze-Lefert, V. Lipka, and S. Somerville Arabidopsis PEN3/PDR8, an ATP Binding Cassette Transporter, Contributes to Nonhost Resistance to Inappropriate Pathogens That Enter by Direct Penetration PLANT CELL, March 1, 2006; 18(3): 731 - 746. [Abstract] [Full Text] [PDF]

				L. A.J. Mur, P. Kenton, R. Atzorn, O. Miersch, and C. Wasternack The Outcomes of Concentration-Specific Interactions between Salicylate and Jasmonate Signaling Include Synergy, Antagonism, and Oxidative Stress Leading to Cell Death Plant Physiology, January 1, 2006; 140(1): 249 - 262. [Abstract] [Full Text] [PDF]

				I.-P. Ahn, S. Kim, and Y.-H. Lee Vitamin B1 Functions as an Activator of Plant Disease Resistance Plant Physiology, July 1, 2005; 138(3): 1505 - 1515. [Abstract] [Full Text] [PDF]

				M. Serrano and P. Guzman Isolation and Gene Expression Analysis of Arabidopsis thaliana Mutants With Constitutive Expression of ATL2, an Early Elicitor-Response RING-H2 Zinc-Finger Gene Genetics, June 1, 2004; 167(2): 919 - 929. [Abstract] [Full Text] [PDF]

				J. E. Lincoln, C. Richael, B. Overduin, K. Smith, R. Bostock, and D. G. Gilchrist Expression of the antiapoptotic baculovirus p35 gene in tomato blocks programmed cell death and provides broad-spectrum resistance to disease PNAS, November 12, 2002; 99(23): 15217 - 15221. [Abstract] [Full Text] [PDF]